PUBLICATION
In vivo molecular toxicity profile of dental bioceramics in embryonic Zebrafish (Danio rerio)
- Authors
- Makkar, H., Verma, S.K., Panda, P.K., Jha, E., Das, B., Mukherjee, K., Suar, M.
- ID
- ZDB-PUB-180731-6
- Date
- 2018
- Source
- Chemical Research in Toxicology 31(9): 914-923 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Aluminum Compounds/toxicity*
- Animals
- Calcium Compounds/toxicity*
- Computer Simulation
- Dental Cements/toxicity*
- Drug Combinations
- Embryo, Nonmammalian/drug effects*
- Female
- Male
- Molecular Docking Simulation
- Oxides/toxicity*
- Silicates/toxicity*
- Zebrafish/embryology*
- PubMed
- 30058326 Full text @ Chem. Res. Toxicol.
Citation
Makkar, H., Verma, S.K., Panda, P.K., Jha, E., Das, B., Mukherjee, K., Suar, M. (2018) In vivo molecular toxicity profile of dental bioceramics in embryonic Zebrafish (Danio rerio). Chemical Research in Toxicology. 31(9):914-923.
Abstract
Investigation of the biocompatibility of potential and commercially available dental material is a major challenge in dental science. This study demonstrates zebrafish model is a novel in vivo model for investigating the biocompatibility of dental materials. Two commercially available dental materials Mineral Trioxide Aggregate (MTA) and Biodentine™ were assessed for their biocompatibility. Biocompatibility analysis was performed in embryonic zebrafish with the help of standard toxicity assays measuring essential parameters like survivability and hatching. Mechanistic and comparative analysis of toxicity was performed by oxidative stress analysis by measuring ROS induction and Apoptosis in zebrafish exposed to dental materials at different concentrations. Molecular investigation at protein level was done by a computational approach using in silico molecular docking and pathway analysis. Toxicity analysis showed a significant reduction in hatching and survivability rate along with morphological malformations with an increase in the concentration of exposed materials. ROS and apoptosis assay results revealed greater biocompatibility of Biodentine™ as compared to MTA which was concentration dependent. In silico analysis showed significant role of tricalcium silicate-protein (Sod1, tp53, RUNX2B) interaction in an exhibition of toxicity. The study provides a new vision and standard in dental material sciences for assessing the biocompatibility of potential novel and commercial available dental materials.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping