PUBLICATION
Effects of calcium and estrogen on the development of the ceratohyal cartilage in zebrafish (Danio rerio) larvae upon embryo and maternal cadmium exposure
- Authors
- Wu, S.M., Su, C.K., Shu, L.H.
- ID
- ZDB-PUB-180731-10
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 213: 47-54 (Journal)
- Registered Authors
- Keywords
- Ceratohyl, Chondrogenesis, Development, Estrogen receptor, Maternal Cd, Teleosts
- MeSH Terms
-
- Animals
- Cadmium/toxicity*
- Calcium/metabolism
- Cartilage/abnormalities
- Cartilage/drug effects*
- Cartilage/embryology
- Cartilage/metabolism
- Chondrogenesis/drug effects*
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Estradiol/metabolism
- Estrogen Receptor alpha/agonists
- Estrogen Receptor alpha/genetics
- Estrogen Receptor alpha/metabolism
- Estrogens/adverse effects
- Female
- Gene Expression Regulation, Developmental/drug effects
- Larva/drug effects
- Larva/growth & development
- Larva/metabolism
- Maternal Exposure/adverse effects*
- Pregnancy
- Receptors, Estrogen/genetics
- Receptors, Estrogen/metabolism
- TRPV Cation Channels/agonists
- TRPV Cation Channels/genetics
- TRPV Cation Channels/metabolism
- Temporal Bone/abnormalities
- Temporal Bone/drug effects
- Temporal Bone/embryology
- Temporal Bone/metabolism
- Teratogens/toxicity
- Water Pollutants, Chemical/toxicity*
- Zebrafish/abnormalities
- Zebrafish/embryology*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zebrafish Proteins/agonists
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 30059766 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Wu, S.M., Su, C.K., Shu, L.H. (2018) Effects of calcium and estrogen on the development of the ceratohyal cartilage in zebrafish (Danio rerio) larvae upon embryo and maternal cadmium exposure. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 213:47-54.
Abstract
The present study is to investigate the reason why the ceratohyal cartilage (CH) angle of zebrafish larvae were larger compared to the control group after their female parents were treated with cadmium (F-Cd). However, the CH angle was smaller compared to the control group when embryos were directly exposed to Cd2+ for 72 h (D-Cd). Results showed that calcium contents of larvae were lower than the control, but the transporter isoforms trpv4 and trpv6 mRNA expressions were significantly increased upon D-Cd treatment. Furthermore, external Ca2+ added during D-Cd treatment reveals that the CH angles of larvae did not appear significantly different compared to the control. On the other hand, E2 (17β-estradiol) contents were higher around 1.9 folds in the ovaries of females; CH angle were over 25°, and Cd2+ contents were higher around 6 folds than the control group on larvae treated through F-Cd treatment; CH angles and E2 levels on larvae were higher than the control after the larvae were treated with 1.84 μM E2 (D-E2); Estradiol receptor (ER) isoforms ERβ1 and ERα mRNA expressions significantly increased when 0 hpf embryos were either treated with D-E2 or D-Cd. According to the results, we suggested that the CH angle of larvae become larger upon F-Cd treatment due to maternal Cd2+ inducing E2 levels. However, the CH angle of larvae appeared to be smaller compared to the control upon D-Cd treatment. We suggested that the CH angle decreased due to the decrease of Ca2+ contents upon Cd2+ exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping