ZFIN ID: ZDB-PUB-180727-9
Nuclear localization of Hif-3α requires two redundant NLS motifs in its unique C-terminal region
Yao, Q., Zhang, P., Lu, L., Liu, Y., Li, Y., Duan, C.
Date: 2018
Source: FEBS letters   592(16): 2769-2775 (Journal)
Registered Authors: Duan, Cunming, Liu, Yunzhang
Keywords: Hypoxia, hypoxia-inducible factor, nuclear localization signal, zebrafish
MeSH Terms:
  • Basic Helix-Loop-Helix Transcription Factors/chemistry*
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Cell Hypoxia
  • Cell Nucleus/metabolism*
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Nuclear Localization Signals
  • Protein Domains
PubMed: 30047986 Full text @ FEBS Lett.
Hif-3α, a member of the hypoxia-inducible factor (HIF) family, enters the nucleus and regulates gene expression in response to hypoxia. The molecular basis of its nuclear localization is not clear. HIF-1α and HIF-2α use a bipartite nuclear localization signal (NLS) to enter the nucleus. This motif is not conserved in Hif-3α. Although there is a conserved Arg/Lys rich motif in the Hif-3α N-terminal region, deletion of this region has minimal effect on Hif-3α nuclear localization. Here, we mapped the functional NLS to the unique C-terminal region of Hif-3α and identified 2 clusters of basic residues critical for its nuclear localization. The two NLS motifs are functionally redundant. Our results, thus, suggest that Hif-3α nuclear localization is mediated through two redundant NLS motifs located in its unique C-terminal region. This article is protected by copyright. All rights reserved.