PUBLICATION
Molecular characterization, ontogeny and expression profiling of mitochondrial antiviral signaling adapter, MAVS from Asian seabass Lates calcarifer, Bloch (1790)
- Authors
- Krishnan, R., Girish Babu, P., Jeena, K., Tripathi, G., Pani Prasad, K.
- ID
- ZDB-PUB-180722-27
- Date
- 2018
- Source
- Developmental and comparative immunology 79: 175-185 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/genetics*
- Animals
- Bass/immunology*
- Cloning, Molecular
- Fish Diseases/immunology*
- Fish Proteins
- Gene Expression Profiling
- Immunity, Innate
- Mitochondria/metabolism*
- Nodaviridae/physiology*
- Phylogeny
- RNA Virus Infections/immunology*
- Signal Transduction
- Spleen/metabolism*
- Zebrafish Proteins/genetics
- PubMed
- 29100916 Full text @ Dev. Comp. Immunol.
Citation
Krishnan, R., Girish Babu, P., Jeena, K., Tripathi, G., Pani Prasad, K. (2018) Molecular characterization, ontogeny and expression profiling of mitochondrial antiviral signaling adapter, MAVS from Asian seabass Lates calcarifer, Bloch (1790). Developmental and comparative immunology. 79:175-185.
Abstract
Mitochondrial antiviral signaling protein (MAVS), an innate immune signaling adapter coordinates the signals received from two independent cytosolic pathogen recognition receptors (RIG-1 and MDA5) to induce antiviral genes. In the present study the MAVS gene of Lates calcarifer (LcMAVS) was cloned and characterized. The complete cDNA sequence of LcMAVS was 3160 bp and encodes a poly peptide of 577 amino acids. Structural analysis of LcMAVS revealed an N-terminal CARD-like domain, central proline-rich domain and a C-terminal transmembrane domain. Phylogenetic analysis indicated that LcMAVS exhibited the closest relationship to P. olivaceous MAVS. LcMAVS was ubiquitously expressed in all tested tissues of healthy fish viz., brain, gill, heart, liver, spleen, kidney and intestine, with highest transcript level in spleen. The mRNA transcript level of LcMAVS in different developmental stages showed constitutive expression in all the stages tested suggesting the maternal transfer of the gene. Significant up regulation in MAVS expression was observed post nervous necrosis virus (NNV) challenge in vivo in all the selected tissues. Further, time course analysis showed that LcMAVS transcripts significantly increased in the brain and spleen tissues after NNV infection. These findings provide useful information for further elucidating the function of LcMAVS in antiviral innate immune response against NNV in Asian seabass.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping