PUBLICATION
Transplantation of Human-Induced Pluripotent Stem Cell-Derived Neural Precursors into Early-Stage Zebrafish Embryos
- Authors
- Strnadel, J., Wang, H., Carromeu, C., Miyanohara, A., Fujimura, K., Blahovcova, E., Nosal, V., Skovierova, H., Klemke, R., Halasova, E.
- ID
- ZDB-PUB-180714-7
- Date
- 2018
- Source
- Journal of molecular neuroscience : MN 65(3): 351-358 (Journal)
- Registered Authors
- Klemke, Richard
- Keywords
- Cell grafting, Human iPS cells, Zebrafish, iPS cell-derived neural precursors
- MeSH Terms
-
- Animals
- Blastocyst/cytology*
- Cells, Cultured
- Humans
- Induced Pluripotent Stem Cells/cytology
- Induced Pluripotent Stem Cells/transplantation*
- Neural Stem Cells/cytology
- Neural Stem Cells/transplantation*
- Stem Cell Transplantation/methods*
- Zebrafish
- PubMed
- 30003430 Full text @ J. Mol. Neurosci.
Citation
Strnadel, J., Wang, H., Carromeu, C., Miyanohara, A., Fujimura, K., Blahovcova, E., Nosal, V., Skovierova, H., Klemke, R., Halasova, E. (2018) Transplantation of Human-Induced Pluripotent Stem Cell-Derived Neural Precursors into Early-Stage Zebrafish Embryos. Journal of molecular neuroscience : MN. 65(3):351-358.
Abstract
Induced pluripotent stem cells (iPS cells) generated from somatic cells through reprogramming hold great promises for regenerative medicine. However, how reprogrammed cells survive, behave in vivo, and interact with host cells after transplantation still remains to be addressed. There is a significant need for animal models that allow in vivo tracking of transplanted cells in real time. In this regard, the zebrafish, a tropical freshwater fish, provides significant advantage as it is optically transparent and can be imaged in high resolution using confocal microscopy. The principal goal of this study was to optimize the protocol for successful short-term and immunosuppression-free transplantation of human iPS cell-derived neural progenitor cells into zebrafish and to test their ability to differentiate in this animal model. To address this aim, we isolated human iPS cell-derived neural progenitor cells from human fibroblasts and grafted them into (a) early (blastocyst)-stage wild-type AB zebrafish embryos or (b) 3-day-old Tg(gfap:GFP) zebrafish embryos (intracranial injection). We found that transplanted human neuronal progenitor cells can be effectively grafted and that they differentiate and survive in zebrafish for more than 2 weeks, validating the model as an ideal platform for in vivo screening experiments. We conclude that zebrafish provides an excellent model for studying iPS cell-derived cells in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping