PUBLICATION
Presence and distribution of leptin and its receptor in the gut of adult zebrafish in response to feeding and fasting
- Authors
- Garcia-Suarez, O., Cabo, R., Abbate, F., Randazzo, B., Laurà, R., Piccione, G., Germanà, A., Levanti, M.
- ID
- ZDB-PUB-180713-16
- Date
- 2018
- Source
- Anatomia, Histologia, Embryologia 47(5): 456-465 (Journal)
- Registered Authors
- Germanà, Antonino
- Keywords
- gut, leptin, zebrafish
- MeSH Terms
-
- Animals
- Fasting/metabolism*
- Gastric Mucosa/metabolism*
- Gene Expression Regulation/genetics
- Immunohistochemistry
- Leptin/genetics*
- Leptin/metabolism*
- Receptors, Leptin/metabolism*
- Stomach/physiology
- Zebrafish
- PubMed
- 29998487 Full text @ Anat. Histol. Embryol.
Citation
Garcia-Suarez, O., Cabo, R., Abbate, F., Randazzo, B., Laurà, R., Piccione, G., Germanà, A., Levanti, M. (2018) Presence and distribution of leptin and its receptor in the gut of adult zebrafish in response to feeding and fasting. Anatomia, Histologia, Embryologia. 47(5):456-465.
Abstract
Leptin is an anorectic hormone secreted mainly by peripheral adipocytes but also by other central and peripheral tissues. It acts by means of a receptor called OB-R, influencing not only appetite and body mass but being also involved in many fields like endocrinology, metabolism and reproduction. Immunohistochemistry and qRT-PCR techniques were, respectively, used to demonstrate the presence of leptin and its receptor in the gut of adult zebrafish and to evaluate the leptin gene expression response to feeding and fasting. Immunoreactivity for the antibodies utilized was demonstrated in feeding but not in fasting fish, and the gene expression analysis corroborates the data obtained by immunohistochemistry. Therefore, all the obtained results support the hypothesis of the role of this hormone in food regulation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping