ZFIN ID: ZDB-PUB-180701-3
Circadian gene period1b regulates proinflammatory cytokine expression through NF-κB signalling in zebrafish
Ren, D.L., Wang, X.B., Hu, B.
Date: 2018
Source: Fish & shellfish immunology   80: 528-533 (Journal)
Registered Authors: Hu, Bing
Keywords: Cytokines, ERK, NF-κB, Zebrafish, per1b
MeSH Terms:
  • Animals
  • Benzamides/pharmacology
  • Circadian Clocks/immunology
  • Cytokines/immunology*
  • Diphenylamine/analogs & derivatives
  • Diphenylamine/pharmacology
  • Embryo, Nonmammalian
  • Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors
  • Extracellular Signal-Regulated MAP Kinases/immunology
  • Inflammation/immunology
  • Larva
  • Mutation
  • NF-kappa B/immunology*
  • Period Circadian Proteins/genetics*
  • Period Circadian Proteins/immunology*
  • Signal Transduction
  • Tail/injuries
  • Zebrafish/genetics*
  • Zebrafish/immunology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology*
PubMed: 29958979 Full text @ Fish Shellfish Immunol.
The circadian clock plays a critical role in regulating the immune system. Our previous publication revealed that a mutation in the circadian gene period1b (per1b) in zebrafish significantly decreased proinflammatory gene expression, particularly under constant darkness (DD) conditions; however, the underlying mechanisms remain unclear. In this study, using per1b-null mutant zebrafish and a larval tail fin injury model, we observed that the loss of per1b resulted in the downregulation expression of proinflammatory cytokines, such as IL-6 and TNF-α, at protein level. Furthermore, the loss of per1b downregulated ERK phosphorylation and inhibited p65 phosphorylation, leading to reduced NF-κB activation, which could downregulate the expression of proinflammatory cytokines, such as IL-6 and TNF-α, in zebrafish. These results provided insight into the communication between the circadian clock and immune functions.