PUBLICATION
The Ability of Different Ketohexoses to Alter Apo-A-I Structure and Function In Vitro and to Induce Hepatosteatosis, Oxidative Stress, and Impaired Plasma Lipid Profile in Hyperlipidemic Zebrafish
- Authors
- Yadav, D., Kim, S.J., Bae, M.A., Kim, J.R., Cho, K.H.
- ID
- ZDB-PUB-180629-3
- Date
- 2018
- Source
- Oxidative medicine and cellular longevity 2018: 3124364 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Apolipoprotein A-I/metabolism*
- Cholesterol
- Hexoses/pharmacology
- Hexoses/therapeutic use*
- Hypercholesterolemia/genetics*
- Hypercholesterolemia/metabolism
- Hyperlipidemias/genetics*
- Hyperlipidemias/metabolism
- Iron Chelating Agents/pharmacology
- Iron Chelating Agents/therapeutic use*
- Oxidative Stress
- Zebrafish
- PubMed
- 29951163 Full text @ Oxid Med Cell Longev
Citation
Yadav, D., Kim, S.J., Bae, M.A., Kim, J.R., Cho, K.H. (2018) The Ability of Different Ketohexoses to Alter Apo-A-I Structure and Function In Vitro and to Induce Hepatosteatosis, Oxidative Stress, and Impaired Plasma Lipid Profile in Hyperlipidemic Zebrafish. Oxidative medicine and cellular longevity. 2018:3124364.
Abstract
In the current study, we have tested the nonenzymatic glycation activities of ketohexoses, such as tagatose and psicose. Although tagatose-treated apoA-I (t-A-I) and psicose-treated apoA-I (p-A-I) exerted more inhibitory activity you cupric ion-mediated low-density lipoprotein (LDL) oxidation and oxidized LDL (oxLDL) phagocytosis into macrophage than fructose-treated apoA-I (f-A-I). In the lipid-free state, t-A-I and f-A-I showed more multimerized band without crosslinking. Since t-A-I lost its phospholipid binding ability, the rHDL formation was not as successful as f-A-I. However, injecting t-A-I showed more antioxidant activities in zebrafish embryo under the presence of oxLDL. Three weeks of consumption of fructose (50% of wt in Tetrabit/4% cholesterol) showed a 14% elevation of serum triacylglycerol (TG), while tagatose-administered group showed 30% reduction in serum TG compared to high cholesterol control. Fructose-fed group showed the biggest area of Oil Red O staining with the intensity as strong as the HCD control. However, tagatose-consumed group showed much lesser Oil Red O-stained area with the reduction of lipid accumulation. In conclusion, although tagatose treatment caused modification of apoA-I, the functional loss was not as much severe as the fructose treatment in macrophage cell model, zebrafish embryo, and hypercholesterolemic zebrafish model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping