|ZFIN ID: ZDB-PUB-180628-2|
Bsx controls pineal complex development
Schredelseker, T., Driever, W.
|Source:||Development (Cambridge, England) 145(13): (Journal)|
|Registered Authors:||Driever, Wolfgang, Schredelseker, Theresa|
|Keywords:||Brain asymmetry, Brain-specific homeobox transcription factor, Bsx, Epithalamus, Habenula, Melatonin, Parapineal organ, Photoreceptors, Pineal gland|
|PubMed:||29945867 Full text @ Development|
Schredelseker, T., Driever, W. (2018) Bsx controls pineal complex development. Development (Cambridge, England). 145(13):.
ABSTRACTNeuroendocrine cells in the pineal gland release melatonin during the night and, in teleosts, are directly photoreceptive. During development of the pineal complex, a small number of cells migrate leftward away from the pineal anlage to form the parapineal cell cluster, a process that is crucial for asymmetrical development of the bilateral habenular nuclei. Here, we show that, throughout zebrafish embryonic development, the brain-specific homeobox (bsx) gene is expressed in all cell types of the pineal complex. We identified Bmp and Noto/Flh as major regulators of bsx expression in the pineal complex. Upon loss of Bsx through the generation of a targeted mutation, embryos fail to form a parapineal organ and develop right-isomerized habenulae. Crucial enzymes in the melatonin biosynthesis pathway are not expressed, suggesting the absence of melatonin from the pineal gland in bsx mutants. Several genes involved in rod-like or cone-like phototransduction are also abnormally expressed, indicating that Bsx has a pivotal role in the differentiation of multiple cell types in the zebrafish pineal complex.