PUBLICATION
Identification and High-Resolution Imaging of α-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry
- Authors
- Bruinen, A.L., Fisher, G.L., Balez, R., van der Sar, A.M., Ooi, L., Heeren, R.M.A.
- ID
- ZDB-PUB-180628-13
- Date
- 2018
- Source
- Journal of the American Society for Mass Spectrometry 29(8): 1571-1581 (Journal)
- Registered Authors
- van der Sar, Astrid M.
- Keywords
- Mass spectrometry imaging, TOF-SIMS, Tandem MS, Vitamin E, α-tocopherol
- MeSH Terms
-
- Animals
- Cells, Cultured
- Fishes
- Humans
- Imaging, Three-Dimensional
- Induced Pluripotent Stem Cells/chemistry
- Male
- Middle Aged
- Molecular Imaging/methods*
- Neurons/chemistry
- Tandem Mass Spectrometry/methods*
- Whole Body Imaging/methods
- Zebrafish
- alpha-Tocopherol/analysis*
- PubMed
- 29949055 Full text @ J. Am. Soc. Mass Spectrom.
Citation
Bruinen, A.L., Fisher, G.L., Balez, R., van der Sar, A.M., Ooi, L., Heeren, R.M.A. (2018) Identification and High-Resolution Imaging of α-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry. 29(8):1571-1581.
Abstract
A unique method for identification of biomolecular components in different biological specimens, while preserving the capability for high speed 2D and 3D molecular imaging, is employed to investigate cellular response to oxidative stress. The employed method enables observing the distribution of the antioxidant α-tocopherol and other molecules in cellular structures via time-of-flight secondary ion mass spectrometry (TOF-SIMS (MS1)) imaging in parallel with tandem mass spectrometry (MS2) imaging, collected simultaneously. The described method is employed to examine a network formed by neuronal cells differentiated from human induced pluripotent stem cells (iPSCs), a model for investigating human neurons in vitro. The antioxidant α-tocopherol is identified in situ within different cellular layers utilizing a 3D TOF-SIMS tandem MS imaging analysis. As oxidative stress also plays an important role in mediating inflammation, the study was expanded to whole body tissue sections of M. marinum-infected zebrafish, a model organism for tuberculosis. The TOF-SIMS tandem MS imaging results reveal an increased presence of α-tocopherol in response to the pathogen. Graphical Abstract ᅟ.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping