Pituitary adenylate cyclase-activating polypeptide (PACAP-38) plays an inhibitory role against inflammation induced by chemical damage to zebrafish hair cells

Kasica-Jarosz, N., Podlasz, P., Kaleczyc, J.
PLoS One   13: e0198180 (Journal)
Registered Authors
Podlasz, Piotr
MeSH Terms
  • Activating Transcription Factor 3/biosynthesis
  • Activating Transcription Factor 3/genetics
  • Animals
  • Animals, Genetically Modified
  • Anti-Inflammatory Agents/pharmacology
  • Copper Sulfate/toxicity
  • Cytokines/biosynthesis
  • Cytokines/genetics
  • Gene Expression Regulation/drug effects
  • Inflammation
  • Larva
  • Lateral Line System/cytology
  • Lateral Line System/drug effects
  • Lateral Line System/metabolism*
  • Mechanoreceptors/drug effects*
  • Mechanoreceptors/metabolism
  • Necrosis
  • Neutrophil Infiltration/drug effects
  • Neutrophils/drug effects
  • Pituitary Adenylate Cyclase-Activating Polypeptide/pharmacology
  • Pituitary Adenylate Cyclase-Activating Polypeptide/physiology*
  • RNA, Messenger/biosynthesis
  • RNA, Messenger/genetics
  • Receptors, Cytokine/biosynthesis
  • Receptors, Cytokine/genetics
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide/biosynthesis
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide/genetics
  • Up-Regulation/drug effects
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
29856797 Full text @ PLoS One
Pituitary adenylate cyclase-activating polypeptide (PACAP-38) is a common neuropeptide exerting a wide spectrum of functions in many fields, including immunology. In the present study, 5-day post-fertilization (dpf) zebrafish larvae of three diverse genetic lines [transgenic lines Tg(MPX:GFP) with GFP-labelled neutrophils and Tg(pou4f3:GAP-GFP) with GFP-labelled hair cells and the wild-type Tuebingen] were used to investigate an inhibitory role of PACAP-38 in inflammation associated with damaged hair cells of the lateral line. Individuals of each genetic line were assigned to four groups: (1) control, and those consisting of larvae exposed to (2) 10 µM CuSO4, (3) 10 µM CuSO4+100 nM PACAP-38 and (4) 100 nM PACAP-38, respectively. Forty-minute exposure to CuSO4 solution was applied to evoke necrosis of hair cells and consequent inflammation. The inhibitory role of PACAP-38 was investigated in vivo under a confocal microscope by counting neutrophils migrating towards damaged hair cells in Tg(MPX:GFP) larvae. In CuSO4-treated individuals, the number of neutrophils associated with hair cells was dramatically increased, while PACAP-38 co-treatment resulted in its over 2-fold decrease. However, co-treatment with PACAP-38 did not prevent hair cells from extensive necrosis, which was found in Tg(pou4f3:GAP-GFP) individuals. Real-Time PCR analysis performed in wild-type larvae demonstrated differential expression pattern of stress and inflammation inducible markers. The most significant findings showed that CuSO4 exposure up-regulated the expression of IL-8, IL-1β, IL-6 and ATF3, while after PACAP-38 co-treatment expression levels of these genes were significantly decreased. The presence of transcripts for all PACAP receptors in neutrophils was also revealed. Adcyap1r1a and vipr1b appeared to be predominant forms. The present results suggest that PACAP-38 should be considered as a factor playing an important regulatory role in inflammatory response associated with pathological processes affecting zebrafish hair cells and it cannot be excluded that this interesting property has more universal significance.
Genes / Markers
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes