PUBLICATION
Sodium benzoate induced developmental defects, oxidative stress and anxiety-like behaviour in zebrafish larva
- Authors
- Gaur, H., Purushothaman, S., Pullaguri, N., Bhargava, Y., Bhargava, A.
- ID
- ZDB-PUB-180531-2
- Date
- 2018
- Source
- Biochemical and Biophysical Research Communications 502(3): 364-369 (Journal)
- Registered Authors
- Keywords
- Anxiety, Gsr, Hatching, Mortality, Sodium benzoate, Zebrafish
- MeSH Terms
-
- Animals
- Anxiety/chemically induced
- Behavior, Animal/drug effects
- Dose-Response Relationship, Drug
- Food Preservatives/administration & dosage
- Food Preservatives/toxicity*
- Glutathione Reductase/genetics
- Lactoylglutathione Lyase/genetics
- Larva/drug effects*
- Larva/growth & development
- Larva/physiology
- Models, Animal
- Oxidative Stress/drug effects
- Sodium Benzoate/administration & dosage
- Sodium Benzoate/toxicity*
- Up-Regulation/drug effects
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- PubMed
- 29842881 Full text @ Biochem. Biophys. Res. Commun.
Citation
Gaur, H., Purushothaman, S., Pullaguri, N., Bhargava, Y., Bhargava, A. (2018) Sodium benzoate induced developmental defects, oxidative stress and anxiety-like behaviour in zebrafish larva. Biochemical and Biophysical Research Communications. 502(3):364-369.
Abstract
Sodium benzoate (SB) is a common food preservative. Its FDA described safety limit is 1000 ppm. Lately, increased use of SB has prompted investigations regarding its effects on biological systems. Data regarding toxicity of SB is divergent and controversial with studies reporting both harmful and beneficial effects. Therefore, we did a systematic dose dependent toxicity study of SB using zebrafish vertebrate animal model. We also investigated oxidative stress and anxiety-like behaviour in zebrafish larva treated with SB. Our results indicate that SB induced developmental (delayed hatching), morphological (pericardial edema, yolk sac edema and tail bending), biochemical (oxidative stress) and behavioural (anxiety-like behaviour) abnormalities in developing zebrafish larva. LC50 of SB induced toxicity was approximately 400 ppm after 48 h of SB exposure. Our study strongly supports its harmful effects on vertebrates at increasing doses. Thus, we suggest caution in the excessive use of this preservative in processed and convenience foods.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping