PUBLICATION
Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide Nanoparticles
- Authors
- Zheng, M., Lu, J., Zhao, D.
- ID
- ZDB-PUB-180530-4
- Date
- 2018
- Source
- Scientific Reports 8: 8083 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Carboxymethylcellulose Sodium/toxicity*
- Environmental Exposure/analysis*
- Environmental Monitoring/methods
- Exome Sequencing
- Female
- Ferrous Compounds/chemistry
- Ferrous Compounds/toxicity*
- Gene Expression Profiling/methods
- Male
- Nanoparticles/chemistry
- Nanoparticles/toxicity*
- Sequence Analysis, RNA/methods
- Toxicity Tests
- Transcriptome/drug effects*
- Zebrafish*/genetics
- Zebrafish*/growth & development
- PubMed
- 29795396 Full text @ Sci. Rep.
Citation
Zheng, M., Lu, J., Zhao, D. (2018) Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide Nanoparticles. Scientific Reports. 8:8083.
Abstract
Increasing utilization of stabilized iron sulfides (FeS) nanoparticles implies an elevated release of the materials into the environment. To understand potential impacts and underlying mechanisms of nanoparticle-induced stress, we used the transcriptome sequencing (RNA-seq) technique to characterize the transcriptomes from adult zebrafish exposed to 10 mg/L carboxymethyl cellulose (CMC) stabilized FeS nanoparticles for 96 h, demonstrating striking differences in the gene expression profiles in liver. The exposure caused significant expression alterations in genes related to immune and inflammatory responses, detoxification, oxidative stress and DNA damage/repair. The complement and coagulation cascades Kyoto encyclopedia of genes and genomes (KEGG) pathway was found significantly up-regulated under nanoparticle exposure. The quantitative real-time polymerase chain reaction using twelve genes confirmed the RNA-seq results. We identified several candidate genes commonly regulated in liver, which may serve as gene indicators when exposed to the nanoparticles. Hepatic inflammation was further confirmed by histological observation of pyknotic nuclei, and vacuole formation upon exposure. Tissue accumulation tests showed a 2.2 times higher iron concentration in the fish tissue upon exposure. This study provides preliminary mechanistic insights into potential toxic effects of organic matter stabilized FeS nanoparticles, which will improve our understanding of the genotoxicity caused by stabilized nanoparticles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping