PUBLICATION
Whole snake venoms: Cytotoxic, anti-metastatic and antiangiogenic properties
- Authors
- Kerkkamp, H., Bagowski, C., Kool, J., van Soolingen, B., Vonk, F., Vlecken, D.
- ID
- ZDB-PUB-180516-11
- Date
- 2018
- Source
- Toxicon : official journal of the International Society on Toxinology 150: 39-49 (Journal)
- Registered Authors
- Bagowski, Christoph P.
- Keywords
- Anti-angiogenic, Anti-metastatic, Cobra, Cytotoxicity, Pancreatic tumour cells, Snake venom, Viper, Zebrafish
- MeSH Terms
-
- Animals
- Antineoplastic Agents/chemistry
- Antineoplastic Agents/pharmacology*
- Cell Line, Tumor
- Cell Movement/drug effects
- Cell Survival
- Elapid Venoms/chemistry
- Elapid Venoms/pharmacology*
- Elapidae
- Embryo, Nonmammalian/drug effects
- Humans
- L-Lactate Dehydrogenase
- Neovascularization, Pathologic/drug therapy*
- Snake Venoms
- Viper Venoms/chemistry
- Viper Venoms/pharmacology*
- Viperidae
- Zebrafish
- PubMed
- 29763628 Full text @ Toxicon.
Citation
Kerkkamp, H., Bagowski, C., Kool, J., van Soolingen, B., Vonk, F., Vlecken, D. (2018) Whole snake venoms: Cytotoxic, anti-metastatic and antiangiogenic properties. Toxicon : official journal of the International Society on Toxinology. 150:39-49.
Abstract
Currently, biological and organic substances are screened in order to find a new generation of therapeutics active against cancer. Previous research has identified promising candidate peptides in snake venom. In this study, venoms from different snake species (Naja annulifera, Naja kaouthia, Ophiophagus hannah and Echis carinatus) were screened for potential anti-cancer properties using pancreatic tumour cells as the assay system. The cells were incubated with venom and then subjected to the following analyses: (i) in vitro cell death (ii) in vitro migration (iii) in vivo dissemination and (iv) in vivo angiogenesis. For the in vivo assays, the cells, after incubation and labelling, were transplanted into the yolk sac of zebrafish embryos for motility and angiogenesis. The results showed strong effects in cells treated with venoms from Ophiophagus hannah and Echis carinatus in the in vitro assays. In the in vivo assays, venom derived from Ophiophagus hannah had the most potent effects with respect to angiogenesis. These venoms might therefore be considered as candidates for further studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping