PUBLICATION
Effects of exposure to BPF on development and sexual differentiation during early life stages of zebrafish (Danio rerio)
- Authors
- Yang, Q., Yang, X., Liu, J., Chen, Y., Shen, S.
- ID
- ZDB-PUB-180515-12
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 210: 44-56 (Journal)
- Registered Authors
- Keywords
- Aromatase, BPA analogues, Bisphenol F (BPF), Sex determination, Sex differentiation, Zebrafish embryos/larvae
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects*
- Endocrine Disruptors/toxicity*
- Female
- Gene Expression Regulation, Developmental/drug effects
- Larva/drug effects*
- Larva/growth & development
- Larva/metabolism
- Male
- Osmolar Concentration
- Ovary/drug effects
- Ovary/embryology
- Ovary/growth & development
- Ovary/metabolism
- Ovum/drug effects
- Ovum/growth & development
- Ovum/metabolism
- Phenols/toxicity*
- Random Allocation
- Sex Determination Processes/drug effects*
- Sex Differentiation/drug effects*
- Testis/drug effects
- Testis/embryology
- Testis/growth & development
- Testis/metabolism
- Toxicity Tests, Acute
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 29758382 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Yang, Q., Yang, X., Liu, J., Chen, Y., Shen, S. (2018) Effects of exposure to BPF on development and sexual differentiation during early life stages of zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 210:44-56.
Abstract
Bisphenol F (BPF) has become a predominant bisphenol contaminant in recent years. It has significant estrogenic properties in both in vivo and in vitro studies. We have previously studied the disrupting mechanisms of BPF on the hypothalamic-pituitary-gonadal axis of adult zebrafish. However, the effects of BPF exposure on development and sexual differentiation of zebrafish embryos/larvae remain unclear. To determine the effects of BPF on the critical stage of sex differentiation in zebrafish, zebrafish embryos/larvae were exposed to 1, 10, 100, and 1000 μg/L BPF from fertilization to 60 days post-fertilization (dpf). Developmental malformations were induced by exposure to BPF from 2 h post-fertilization (hpf), with a LC50 of 10,030 μg/L at 96 hpf and 9391 μg/L at 120 hpf. Long-term exposure during sex differentiation tended to result in a female sex ratio bias. Histological analyses at 60 dpf indicated that the development of ovo-testes and immature ovaries was induced by 100 and 1000 μg/L BPF. Homogenate testosterone levels decreased and 17β-estradiol levels increased in zebrafish in a concentration-dependent manner. BPF exposure suppressed gene expression of double sex, Mab3-related transcription factor 1(dmrt1), fushi tarazu factor 1d (ff1d), sry-box containing gene 9a (sox9a) and anti-Mullerian hormone (amh); induced expression of the forkhead box L2 transcription factor (foxl2), leading to increased expression of aromatase (cyp19a1a), which promoted production of estrogens, and further caused phenotypic feminization of zebrafish. These results suggest that developmental exposure to BPF has adverse effects on sexual differentiation, and the results were useful for a BPF risk assessment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping