PUBLICATION
Behavioral and biochemical effects of ethanol withdrawal in zebrafish
- Authors
- da Silva Chaves, S.N., Rocha, G.F., Costa, B.P.D., de Oliveira, W.E.A., Lima, M.G., de Siqueira Silva, D.H., Maximino, C.
- ID
- ZDB-PUB-180426-5
- Date
- 2018
- Source
- Pharmacology, biochemistry, and behavior 169: 48-58 (Journal)
- Registered Authors
- Maximino, Caio
- Keywords
- Anxiety, Catalase activity, Danio rerio, Epileptic seizures, Ethanol withdrawal
- MeSH Terms
-
- Animals
- Anxiety/etiology
- Brain/enzymology
- Catalase/metabolism
- Darkness
- Ethanol/adverse effects*
- Meta-Analysis as Topic
- Models, Biological
- Pilocarpine/pharmacology
- Risk Assessment
- Seizures/chemically induced
- Substance Withdrawal Syndrome/metabolism*
- Substance Withdrawal Syndrome/psychology*
- Zebrafish/physiology*
- PubMed
- 29689295 Full text @ Pharmacol. Biochem. Behav.
Citation
da Silva Chaves, S.N., Rocha, G.F., Costa, B.P.D., de Oliveira, W.E.A., Lima, M.G., de Siqueira Silva, D.H., Maximino, C. (2018) Behavioral and biochemical effects of ethanol withdrawal in zebrafish. Pharmacology, biochemistry, and behavior. 169:48-58.
Abstract
Chronic alcohol use induces adaptations and toxicity that can induce symptoms of anxiety, autonomic hyperarousal, and epileptic seizures when alcohol is removed (withdrawal syndrome). Zebrafish has recently gained wide attention as a behavioral model to study the neurobehavioral effects of acute and chronic alcohol use, including withdrawal. The literature, however, is very contradictory on findings regarding withdrawal effects, with some studies reporting increased anxiety, while others report no effect. A meta-analytic approach was taken to find the sources of this heterogeneity, and ethanol concentration during exposure and exposure duration were found to be the main sources of variation. A conceptual replication was also made using continuous exposure for 16 days in waterborne ethanol (0.5%) and assessing anxiety-like behavior in the light/dark test after 60 min withdrawal. Withdrawal was shown to reduce preference for darkness, consistent with decreased anxiety, but to increase risk assessment, consistent with increased anxiety. Animals were also subjected to the withdrawal protocol and injected with pilocarpine in a sub-convulsive dose to assess susceptibility to epileptic seizure-like behavior. The protocol was sufficient to increase susceptibility to epileptic seizure-like behavior in animals exposed to ethanol. Finally, withdrawal also decreased catalase activity in the brain, but not in the head kidney, suggesting mechanisms associated with the behavioral effects of ethanol withdrawal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping