PUBLICATION
Anti-inflammatory effect of Apo-9'-fucoxanthinone via inhibition of MAPKs and NF-kB signaling pathway in LPS-stimulated RAW 264.7 macrophages and zebrafish model
- Authors
- Kim, E.A., Kim, S.Y., Ye, B.R., Kim, J., Ko, S.C., Lee, W.W., Kim, K.N., Choi, I.W., Jung, W.K., Heo, S.J.
- ID
- ZDB-PUB-180422-9
- Date
- 2018
- Source
- International Immunopharmacology 59: 339-346 (Journal)
- Registered Authors
- Keywords
- Anti-inflammation, Apo-9′-fucoxanthinone, Cytokines, Macrophage, Seaweed, Zebrafish
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/pharmacology*
- Cyclooxygenase 2/genetics
- Cyclooxygenase 2/metabolism
- Cytokines/genetics
- Dinoprostone/metabolism
- Embryo, Nonmammalian
- Lipopolysaccharides/pharmacology
- Mice
- Mitogen-Activated Protein Kinases/antagonists & inhibitors*
- Mitogen-Activated Protein Kinases/metabolism
- NF-kappa B/antagonists & inhibitors*
- NF-kappa B/metabolism
- Nitric Oxide/metabolism
- Nitric Oxide Synthase Type II/genetics
- Nitric Oxide Synthase Type II/metabolism
- RAW 264.7 Cells
- Reactive Oxygen Species/metabolism
- Signal Transduction/drug effects
- Terpenes/pharmacology*
- Zebrafish
- PubMed
- 29679858 Full text @ Int. Immunopharmacol.
Citation
Kim, E.A., Kim, S.Y., Ye, B.R., Kim, J., Ko, S.C., Lee, W.W., Kim, K.N., Choi, I.W., Jung, W.K., Heo, S.J. (2018) Anti-inflammatory effect of Apo-9'-fucoxanthinone via inhibition of MAPKs and NF-kB signaling pathway in LPS-stimulated RAW 264.7 macrophages and zebrafish model. International Immunopharmacology. 59:339-346.
Abstract
In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In lipopolysaccharide (LPS)-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of inducible nitric oxide synthase (iNOS), suppressed cyclooxygenase-2 (COX-2) and an inflammatory cytokines; IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, prostaglandin E2 (PGE2), and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 μg/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping