PUBLICATION
Possible mechanisms of prednisolone-induced osteoporosis in zebrafish larva
- Authors
- He, H., Wang, C., Tang, Q., Yang, F., Xu, Y.
- ID
- ZDB-PUB-180418-12
- Date
- 2018
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 101: 981-987 (Journal)
- Registered Authors
- Yang, Fan
- Keywords
- ECM, GIOP, Osteoblast, Osteoclast
- MeSH Terms
-
- Animals
- Extracellular Matrix/genetics
- Extracellular Matrix/metabolism
- Gene Expression Regulation/drug effects
- Larva/drug effects
- Osteoblasts/drug effects
- Osteoblasts/metabolism
- Osteoporosis/chemically induced*
- Osteoporosis/pathology*
- Phenotype
- Prednisolone/adverse effects*
- Zebrafish/metabolism*
- PubMed
- 29635908 Full text @ Biomed. Pharmacother.
Citation
He, H., Wang, C., Tang, Q., Yang, F., Xu, Y. (2018) Possible mechanisms of prednisolone-induced osteoporosis in zebrafish larva. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 101:981-987.
Abstract
Glucocorticoid-induced osteoporosis (GIOP) is a serious clinical bone disease that results from the long-term consumption of glucocorticoids or glucocorticoid-like drugs. Although many studies have attempted to determine the mechanisms of GIOP, they are still unclear. In this study, we established a zebrafish model of glucocorticoid-like drug-induced osteoporosis by treating larvae with prednisolone. We then quantified the expression of a selection of extracellular matrix (ECM)-, osteoblast-, and osteoclast-related genes. Our results showed that at 15 days post fertilization, zebrafish larvae treated with 25 μM prednisolone are a suitable model for GIOP, not only owing to the decrease in robust bone mass but also because of significant alterations in gene expression. The quantification of the expression of ECM-, osteoblast-, and osteoclast- related genes revealed that mmp9 and mmp13 were significantly upregulated and entpd5a, acp5a, and sost were significantly downregulated. These genes may be a target for future research into GIOP. Our study thus provides new insights into GIOP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping