ZFIN ID: ZDB-PUB-180407-3
Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish
Janjuha, S., Singh, S.P., Tsakmaki, A., Mousavy-Gharavy, N., Murawala, P., Konantz, J., Birke, S., Hodson, D.J., Rutter, G., Bewick, G., Ninov, N.N.
Date: 2018
Source: eLIFE 7: (Journal)
Registered Authors: Ninov, Nikolay, Singh, Sumeet Pal
Keywords: cell biology, zebrafish
Microarrays: GEO:GSE106938
MeSH Terms: none
PubMed: 29624168 Full text @ Elife
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ABSTRACT
The pancreatic islet, a cellular community harboring the insulin-producing beta-cells, is known to undergo age-related alterations. However, only a handful of signals associated with aging have been identified. By comparing beta-cells from younger and older zebrafish, here we show that the aging islets exhibit signs of chronic inflammation. These include recruitment of tnfα-expressing macrophages and the activation of NF-kB signaling in beta-cells. Using a transgenic reporter, we show that NF-kB activity is undetectable in juvenile beta-cells, whereas cells from older fish exhibit heterogeneous NF-kB activity. We link this heterogeneity to differences in gene expression and proliferation. Beta-cells with high NF-kB signaling proliferate significantly less compared to their neighbors with low activity. The NF-kB signalinghi cells also exhibit premature upregulation of socs2, an age-related gene that inhibits beta-cell proliferation. Together, our results show that NF-kB activity marks the asynchronous decline in beta-cell proliferation with advancing age.
ADDITIONAL INFORMATIONNo data available