PUBLICATION
Zebrafish: A Pharmacogenetic Model for Anesthesia
- Authors
- Bedell, V., Buglo, E., Marcato, D., Pylatiuk, C., Mikut, R., Stegmaier, J., Scudder, W., Wray, M., Züchner, S., Strähle, U., Peravali, R., Dallman, J.E.
- ID
- ZDB-PUB-180329-3
- Date
- 2018
- Source
- Methods in enzymology 602: 189-209 (Chapter)
- Registered Authors
- Bedell, Victoria, Dallman, Julia, Mikut, Ralf, Peravali, Ravindra, Pylatiuk, Christian, Strähle, Uwe
- Keywords
- Anesthesia, Behavior, Genome editing, Propofol, Zebrafish
- MeSH Terms
-
- Anesthesia/adverse effects
- Anesthesia/methods*
- Anesthetics/administration & dosage
- Anesthetics/adverse effects
- Anesthetics/pharmacokinetics*
- Animals
- Animals, Genetically Modified/genetics
- Behavior, Animal/drug effects
- Biotransformation/genetics
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Drug Discovery/methods
- Gene Editing/methods
- Gene Knockdown Techniques/instrumentation
- Gene Knockdown Techniques/methods
- High-Throughput Screening Assays/instrumentation
- High-Throughput Screening Assays/methods*
- Humans
- Mutation
- Pharmacogenetics/methods*
- Pharmacogenomic Variants/genetics
- Propofol/administration & dosage
- Propofol/adverse effects
- Propofol/pharmacokinetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 29588029 Full text @ Methods Enzymol.
Citation
Bedell, V., Buglo, E., Marcato, D., Pylatiuk, C., Mikut, R., Stegmaier, J., Scudder, W., Wray, M., Züchner, S., Strähle, U., Peravali, R., Dallman, J.E. (2018) Zebrafish: A Pharmacogenetic Model for Anesthesia. Methods in enzymology. 602:189-209.
Abstract
General anesthetics are small molecules that interact with and effect the function of many different proteins to promote loss of consciousness, amnesia, and sometimes, analgesia. Owing to the complexity of this state transition and the transient nature of these drug/protein interactions, anesthetics can be difficult to study. The zebrafish is an emerging model for the discovery of both new genes required for the response to and side effects of anesthesia. Here we discuss the tools available to manipulate the zebrafish genome, including both genetic screens and genome engineering approaches. Additionally, there are various robust behavior assays available to study anesthetic and other drug responses. These assays are available for single-gene study or high throughput for genetic or drug discovery. Finally, we present a case study of using propofol as an anesthetic in the zebrafish. These techniques and protocols make the zebrafish a powerful model to study anesthetic mechanisms and drug discovery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping