PUBLICATION

Zebrafish: A Pharmacogenetic Model for Anesthesia

Authors
Bedell, V., Buglo, E., Marcato, D., Pylatiuk, C., Mikut, R., Stegmaier, J., Scudder, W., Wray, M., Züchner, S., Strähle, U., Peravali, R., Dallman, J.E.
ID
ZDB-PUB-180329-3
Date
2018
Source
Methods in enzymology   602: 189-209 (Chapter)
Registered Authors
Bedell, Victoria, Dallman, Julia, Mikut, Ralf, Peravali, Ravindra, Pylatiuk, Christian, Strähle, Uwe
Keywords
Anesthesia, Behavior, Genome editing, Propofol, Zebrafish
MeSH Terms
  • High-Throughput Screening Assays/instrumentation
  • High-Throughput Screening Assays/methods*
  • Humans
  • Pharmacogenetics/methods*
  • Anesthetics/administration & dosage
  • Anesthetics/adverse effects
  • Anesthetics/pharmacokinetics*
  • Gene Knockdown Techniques/instrumentation
  • Gene Knockdown Techniques/methods
  • Anesthesia/adverse effects
  • Anesthesia/methods*
  • Mutation
  • Disease Models, Animal
  • Pharmacogenomic Variants/genetics
  • Dose-Response Relationship, Drug
  • Drug Discovery/methods
  • Zebrafish/genetics*
  • Animals, Genetically Modified/genetics
  • Propofol/administration & dosage
  • Propofol/adverse effects
  • Propofol/pharmacokinetics
  • Zebrafish Proteins/genetics
  • Animals
  • Behavior, Animal/drug effects
  • Gene Editing/methods
  • Biotransformation/genetics
PubMed
29588029 Full text @ Methods Enzymol.
Abstract
General anesthetics are small molecules that interact with and effect the function of many different proteins to promote loss of consciousness, amnesia, and sometimes, analgesia. Owing to the complexity of this state transition and the transient nature of these drug/protein interactions, anesthetics can be difficult to study. The zebrafish is an emerging model for the discovery of both new genes required for the response to and side effects of anesthesia. Here we discuss the tools available to manipulate the zebrafish genome, including both genetic screens and genome engineering approaches. Additionally, there are various robust behavior assays available to study anesthetic and other drug responses. These assays are available for single-gene study or high throughput for genetic or drug discovery. Finally, we present a case study of using propofol as an anesthetic in the zebrafish. These techniques and protocols make the zebrafish a powerful model to study anesthetic mechanisms and drug discovery.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping