PUBLICATION
Modulation of transcription of genes related to the hypothalamic-pituitary-gonadal and the hypothalamic-pituitary-adrenal axes in zebrafish (Danio rerio) embryos/larvae by androstenedione
- Authors
- Hou, L.P., Shu, H., Lin, L.L., Xu, S.Y., Wu, Y.X., Rong, X.J., Hu, J.J., Song, L.Y., Liang, Y.Q., Chen, H.X., Ying, G.G., Tian, C.E.
- ID
- ZDB-PUB-180328-6
- Date
- 2018
- Source
- Ecotoxicology and environmental safety 156: 403-408 (Journal)
- Registered Authors
- Keywords
- Androstenodione, Embryos/larvae, Hypothalamic-pituitary-adrenal axis, Hypothalamic-pituitary-gonadal axis, Zebrafish
- MeSH Terms
-
- Androstenedione/pharmacology*
- Animals
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Endocrine System/drug effects
- Gene Expression Regulation
- Gonads/drug effects*
- Gonads/metabolism
- Hypothalamo-Hypophyseal System/drug effects*
- Hypothalamo-Hypophyseal System/metabolism
- Larva/drug effects*
- Pituitary Gland/drug effects*
- Pituitary Gland/metabolism
- Pituitary-Adrenal System/drug effects*
- Pituitary-Adrenal System/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reproduction/drug effects
- Transcription, Genetic
- Zebrafish/embryology
- PubMed
- 29587208 Full text @ Ecotoxicol. Environ. Saf.
Citation
Hou, L.P., Shu, H., Lin, L.L., Xu, S.Y., Wu, Y.X., Rong, X.J., Hu, J.J., Song, L.Y., Liang, Y.Q., Chen, H.X., Ying, G.G., Tian, C.E. (2018) Modulation of transcription of genes related to the hypothalamic-pituitary-gonadal and the hypothalamic-pituitary-adrenal axes in zebrafish (Danio rerio) embryos/larvae by androstenedione. Ecotoxicology and environmental safety. 156:403-408.
Abstract
This study aimed to determine the effects of Androstenodione (AED) on the transcriptional expression of genes involved in the hypothalamic-pituitary-gonadal (HPG) and the hypothalamic-pituitary-adrenal (HPA) axes in the zebrafish embryos/larvae. Zebrafish embryos were exposed to 0, 4.0, 45.0, 487.0, and 980.0 ng/L of AED from the day of fertilization to 144 h post fertilization (hpf), during which the transcriptional profiles of key genes related to the HPG and HPA axes were examined daily using quantitative real-time PCR. The AED exposure significantly up-regulated several receptor signaling pathways and the key genes involved in various steps of the steroidogenic pathways were also affected. In addition, the AED exposure could significantly modulate the transcriptional profiles of the other target genes related to hypothalamic and pituitary hormones. The findings of this study suggest that AED, at environmentally relevant concentrations, affects the adrenal endocrine systems and the reproduction of zebrafish by interrupting the HPG and HPA axes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping