PUBLICATION
Ketamine induction of p53-dependent apoptosis and oxidative stress in zebrafish (Danio rerio) embryos
- Authors
- Félix, L.M., Vidal, A.M., Serafim, C., Valentim, A.M., Antunes, L.M., Monteiro, S.M., Matos, M., Coimbra, A.M.
- ID
- ZDB-PUB-180317-15
- Date
- 2018
- Source
- Chemosphere 201: 730-739 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Gene expression, Ketamine, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Apoptosis/drug effects*
- Apoptosis/genetics
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Embryonic Development/genetics
- Gene Expression/drug effects
- Ketamine/toxicity*
- Oxidative Stress/drug effects*
- Oxidative Stress/genetics
- Tumor Suppressor Protein p53/metabolism*
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/embryology
- PubMed
- 29547861 Full text @ Chemosphere
Citation
Félix, L.M., Vidal, A.M., Serafim, C., Valentim, A.M., Antunes, L.M., Monteiro, S.M., Matos, M., Coimbra, A.M. (2018) Ketamine induction of p53-dependent apoptosis and oxidative stress in zebrafish (Danio rerio) embryos. Chemosphere. 201:730-739.
Abstract
Ketamine is a widely used pharmaceutical that has been detected in water sources worldwide. Zebrafish embryos were used in this study to investigate the oxidative stress and apoptotic signals following a 24h exposure to different ketamine concentrations (0, 50, 70 and 90 mg L-1). Early blastula embryos (∼2 h post fertilisation-hpf) were exposed for 24 h and analysed at 8 and 26 hpf. Reactive oxygen species and apoptotic cells were identified in vivo, at 26 hpf. Enzymatic activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE)), glutathione levels (oxidised (GSSG) and reduced (GSH)), oxidative damage (lipid peroxidation (LPO) and protein carbonyls (CO)) as well as oxidative stress (gclc, gstp1, sod1 and cat), apoptosis (casp3a, casp6, casp8, casp9, aifm1 and tp53) and cell proliferation (pcna) related-genes were evaluated at 8 and 26 hpf. Caspase (3 and 9) activity was also determined at both time-points by colorimetric methods. Superoxide dismutase (SOD), catalase (CAT), glutathione levels (GSSG), caspase-9 and reactive oxygen species (ROS) were shown to be affected by ketamine exposure while in vivo analysis showed no difference in ROS. A significant up-regulation of superoxide dismutase (sod1) and catalase (cat) genes expression was also perceived. Ketamine-induced apoptosis was observed in vivo and confirmed by the apoptotic-related genes up-regulation. The overall results suggest that ketamine induced oxidative stress and apoptosis through the involvement of p53-dependent pathways in zebrafish embryos which could be important for the evaluation of the overall risk of ketamine in aquatic environments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping