ZFIN ID: ZDB-PUB-180314-1
Expression profile of glucose transport-related genes under chronic and acute exposure to growth hormone in zebrafish
Dalmolin, C., Almeida, D.V., Figueiredo, M.A., Marins, L.F.
Date: 2018
Source: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology   221: 1-6 (Journal)
Registered Authors: Almeida, Daniela Volcan, Figueiredo, Marcio de Azevedo, Marins, Luis Fernando
Keywords: Danio rerio, GLUT, Growth hormone, Transgenic
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biological Transport
  • Brain/metabolism
  • Energy Metabolism
  • Gene Expression Profiling*
  • Glucose/metabolism*
  • Growth Hormone/administration & dosage*
  • Growth Hormone/genetics
  • Growth Hormone/metabolism*
  • Male
  • Monosaccharide Transport Proteins/genetics*
  • Recombinant Proteins/administration & dosage
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 29530717 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
The brain is a highly demanding organ in terms of energy requirements, and precise regulatory mechanisms must operate to ensure adequate energy delivery to maintain normal neuronal activity. Of the energy-promoting substrates present in the circulation, glucose is preferred by the brain, and as with all other substrates, its utilization depends on the presence of humoral factors such as hormones including growth hormone (GH). Glucose enters the cells though specific transport proteins. Among all transporter families and subtypes described to date, the most studied ones are the glucose transporters (GLUTs). The aim of this study is to determine a possible relationship between GH and GLUTs. Therefore, we evaluated the effect of GH-transgenesis and recombinant GH injections upon GLUT expression in the brain of male zebrafish. Overall, the results demonstrated that increasing the GH concentrations above the normal level, via transgenesis or injection, in the fish may impair energy uptake by the brain. This appeared to occur through downregulation of most of the analyzed GLUTs.