PUBLICATION
Beta-glucan enhances the response to SVCV infection in zebrafish
- Authors
- M Medina-Gali, R., Ortega-Villaizán, M.D.M., Mercado, L., Novoa, B., Coll, J., Perez, L.
- ID
- ZDB-PUB-180311-3
- Date
- 2018
- Source
- Developmental and comparative immunology 84: 307-314 (Journal)
- Registered Authors
- Morales, Julio Coll, Novoa, Beatriz
- Keywords
- Antiviral, Beta-glucan, Immunostimulant, SVCV, Zebrafish
- MeSH Terms
-
- Animals
- Antiviral Agents/metabolism*
- Cell Line
- Cytokines/genetics
- Cytokines/metabolism
- Embryonic Stem Cells/physiology*
- Embryonic Stem Cells/virology
- Fish Diseases/immunology*
- Fish Proteins/metabolism
- Gene Expression Regulation
- Immunity, Innate/genetics
- Rhabdoviridae/immunology*
- Rhabdoviridae Infections/immunology*
- Signal Transduction
- Yeasts/metabolism*
- Zebrafish/immunology*
- Zymosan/metabolism*
- beta-Glucans/metabolism*
- PubMed
- 29524446 Full text @ Dev. Comp. Immunol.
Citation
M Medina-Gali, R., Ortega-Villaizán, M.D.M., Mercado, L., Novoa, B., Coll, J., Perez, L. (2018) Beta-glucan enhances the response to SVCV infection in zebrafish. Developmental and comparative immunology. 84:307-314.
Abstract
The antiviral effects of beta-glucan, an immunostimulatory agent were studied in zebrafish both in vitro and in vivo. Here we show that zebrafish ZF4 cells as well as whole fish primed with yeast β-glucan zymosan exhibited increased cytokine expression and elevated response to spring viremia of carp virus (SVCV) infection. In vitro, previous treatment of β-glucan enhanced ZF4 cell viability against SVCV infection which is associated to the activation of interferon signaling pathway and inflammatory cytokines gene expression. In vivo, the SVCV-infected fish primed with β-glucan had a higher survival rate (≈73%) than the control SVCV-infected group (≈33%). Additionally, up-regulation of the expression of a set of genes involved in innate immune response was detected in zebrafish intraperitoneally injected of β-glucan: il1b, il6, il8, il10 and tnfa transcripts showed increased expression that appear to be rapid (2 days) but not long-lived (less than 2 weeks). The present study is, to our knowledge, the first to combine cell culture and in vivo approaches to describe host response to β-glucan stimulation and viral infection in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping