PUBLICATION
Chitosan nanoparticles: A positive immune response modulator as display in zebrafish larvae against Aeromonas hydrophila infection
- Authors
- Nikapitiya, C., Dananjaya, S.H.S., De Silva, B.C.J., Heo, G.J., Oh, C., De Zoysa, M., Lee, J.
- ID
- ZDB-PUB-180307-10
- Date
- 2018
- Source
- Fish & shellfish immunology 76: 240-246 (Journal)
- Registered Authors
- Keywords
- Aeromonas hydrophila, Chitosan nanoparticles, Immune response, Zebrafish larvae
- MeSH Terms
-
- Aeromonas hydrophila/physiology
- Animals
- Chitosan/administration & dosage
- Chitosan/pharmacology*
- Fish Diseases/immunology*
- Gram-Negative Bacterial Infections/immunology*
- Immunity, Innate*
- Immunologic Factors/administration & dosage
- Immunologic Factors/pharmacology*
- Longevity
- Nanoparticles*/administration & dosage
- Particle Size
- Zebrafish/immunology*
- PubMed
- 29510255 Full text @ Fish Shellfish Immunol.
Citation
Nikapitiya, C., Dananjaya, S.H.S., De Silva, B.C.J., Heo, G.J., Oh, C., De Zoysa, M., Lee, J. (2018) Chitosan nanoparticles: A positive immune response modulator as display in zebrafish larvae against Aeromonas hydrophila infection. Fish & shellfish immunology. 76:240-246.
Abstract
Chitosan nanoparticles (CNPs) were synthesized by ionic gelation method and its immunomodulatory properties were investigated in zebrafish larvae. Average particle size and zeta potential were 181.2 nm and +37.2 mv, respectively. Initially, toxicity profile was tested in zebrafish embryo at 96 h post fertilization (hpf) stage using medium molecular weight chitosan (MMW-C) and CNPs. At 5 μg/mL, the hatching rate was almost similar in both treatments, however, the survival rate was lower in MMW-C compared to CNPs exposure, suggesting that toxicity effect of CNPs in hatched larvae was minimal at 5 μg/mL compared to MMW-C. Quantitative real time PCR results showed that in CNPs exposed larvae at 5 days post fertilization (5 dpf) stage, immune related (il-1β, tnf-α, il-6, il-10, cxcl-18b, ccl34a.4, cxcl-8a, lyz-c, defβl-1, irf-1a, irf-3, MxA) and stress (hsp-70) response genes were induced. In contrast, basal or down regulated expression of antioxidant genes (gstp-1, cat, sod-1, prdx-4, txndr-1) were observed. Moreover, zebrafish larvae (at 5 dpf stage) exposed to CNPs (5 μg/mL) showed higher survival rate at 72 h post infection stage against pathogenic Aeromonas hydrophila challenge compared to controls. These results suggest that although CNPs can have toxic effects to the larvae at higher doses, CNPs exposure at 5 μg/mL could enhance the immune responses and develop the disease resistance against A. hydrophila, which could be attributed to its strong immune modulatory properties.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping