PUBLICATION
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish
- Authors
- Castro, A.J.G., Baptista, I.E., de Moura, K.R.S., Padilha, F., Tonietto, J., de Souza, A.Z.P., Soares, C.H.L., Silva, F.R.M.B., Van Der Kraak, G.
- ID
- ZDB-PUB-180303-14
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 206-207: 41-47 (Journal)
- Registered Authors
- Van Der Kraak, Glen
- Keywords
- Biochemistry, Lactate, Lactate dehydrogenase, Morphology, Oxidative stress, P38 mitogen-activated protein kinase, Testis
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Brazil
- Extraction and Processing Industry*
- Lactate Dehydrogenases/metabolism
- Lactic Acid/metabolism
- Liver/cytology
- Liver/drug effects*
- Liver/metabolism
- MAP Kinase Signaling System/drug effects
- Male
- Oxidative Stress/drug effects*
- Paper*
- Reactive Oxygen Species/metabolism
- Seminiferous Tubules/cytology
- Seminiferous Tubules/drug effects
- Seminiferous Tubules/metabolism
- Testis/cytology
- Testis/drug effects*
- Testis/metabolism
- Toxicity Tests, Acute
- Vacuoles/drug effects
- Vacuoles/metabolism
- Wastewater/toxicity*
- Wood*
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 29499384 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Castro, A.J.G., Baptista, I.E., de Moura, K.R.S., Padilha, F., Tonietto, J., de Souza, A.Z.P., Soares, C.H.L., Silva, F.R.M.B., Van Der Kraak, G. (2018) Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 206-207:41-47.
Abstract
While many studies have shown that pulp mill effluents can affect ovarian physiology in fish, far fewer studies have considered the effects in males. We conducted a lab study to examine the effects of effluent from a Brazilian pulp and paper mill on hepatic and testicular morphology and various aspects of testicular physiology in the zebrafish Danio rerio. Males were exposed to lab water (control) or 4% effluent for 14 days. Effluent exposure did not affect testis size as measured by the gonadosomatic index, but contributed to morphological changes in the seminiferous tubules. The number of cysts with histopathological changes was elevated in effluent-exposed fish and the number of cysts containing spermatids was significantly reduced. The testis of effluent exposed fish had reduced levels of lactate, elevated lactate dehydrogenase activity, increased levels of reactive oxygen species and reduced levels of phosphorylated P38 mitogen-activated protein kinase (pP38 MAPK). Separate studies showed that the addition of lactate to testicular tissue incubated in vitro increased the activation of P38 MAPK. Effluent exposure also increased vacuolization, necrosis, apoptosis, hyperemia, and fat infiltration of the hepatocytes. Collectively, we provide evidence of short term effects of pulp mill effluent on testicular and hepatic physiology and biochemistry in the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping