PUBLICATION
The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes
- Authors
- Bautista, F.E.A., Junior, A.S.V., Corcini, C.D., Acosta, I.B., Caldas, S.S., Primel, E.G., Zanette, J.
- ID
- ZDB-PUB-180223-63
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 206-207: 17-22 (Journal)
- Registered Authors
- Keywords
- RT-qPCR, atrazine, fish, reproduction, sperm
- MeSH Terms
-
- 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/chemistry
- 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/genetics
- 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/metabolism
- Animals
- Atrazine/toxicity*
- Cell Cycle Proteins
- Cystic Fibrosis Transmembrane Conductance Regulator/antagonists & inhibitors
- Cystic Fibrosis Transmembrane Conductance Regulator/genetics
- Cystic Fibrosis Transmembrane Conductance Regulator/metabolism
- Gene Expression Regulation, Developmental/drug effects*
- Glutathione Peroxidase/antagonists & inhibitors
- Glutathione Peroxidase/genetics
- Glutathione Peroxidase/metabolism
- Herbicides/toxicity*
- Male
- Membrane Proteins/antagonists & inhibitors
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Mitochondria/drug effects
- Mitochondria/enzymology
- Mitochondria/metabolism
- Mitochondrial Membranes/drug effects
- Mitochondrial Membranes/enzymology
- Mitochondrial Membranes/metabolism
- Osmolar Concentration
- Random Allocation
- Saccharomyces cerevisiae Proteins
- Sperm Motility/drug effects
- Spermatogenesis/drug effects*
- Superoxide Dismutase/antagonists & inhibitors
- Superoxide Dismutase/genetics
- Superoxide Dismutase/metabolism
- Testis/cytology
- Testis/drug effects*
- Testis/metabolism
- Water Pollutants, Chemical/toxicity*
- Xeroderma Pigmentosum/genetics
- Xeroderma Pigmentosum/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 29471151 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Bautista, F.E.A., Junior, A.S.V., Corcini, C.D., Acosta, I.B., Caldas, S.S., Primel, E.G., Zanette, J. (2018) The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 206-207:17-22.
Abstract
The herbicide atrazine (ATZ) is used worldwide in the control of annual grasses and broad-leaved weeds. The present study evaluated sperm quality parameters in zebrafish Danio rerio after 11-day exposure to nominal ATZ concentrations of 2, 10, and 100 μg L-1. All ATZ concentrations caused a decrease in motility, mitochondrial functionality, and membrane integrity, as measured using conventional microscopy or fluorescence microscopy with specific probes. The DNA integrity of sperm was not affected. The levels of expression of genes related to spermatogenesis, antioxidant defenses, and DNA repair were also investigated using RT-qPCR. The ATZ caused transcriptional repression of the spermatogenesis-related genes SRD5A2 and CFTR, the antioxidant defense genes SOD2 and GPX4B, and the DNA repair gene XPC. This is the first study to show that environmentally relevant concentrations of ATZ significantly affect the sperm quality in fish, possibly resulting in reduced fertility rates. In addition, we showed that the repression of genes related to spermatogenesis and cellular defense could be part of the mechanisms involved in the ATZ toxicity in the testes of male fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping