PUBLICATION

A Simple Method of Cataract Induction in Adult Zebrafish

Authors
Prior, H.M., Letwin, K., Tuininga, A., Nguyen, M.
ID
ZDB-PUB-180214-16
Date
2018
Source
Zebrafish   15(2): 211-212 (Journal)
Registered Authors
Prior, Heather
Keywords
cataract, hydrogen peroxide, induction, lens
MeSH Terms
  • Animals
  • Cataract/chemically induced*
  • Cataract/pathology
  • Disease Models, Animal*
  • Hydrogen Peroxide
  • Lens, Crystalline/drug effects
  • Lens, Crystalline/pathology*
  • Oxidants
  • Zebrafish*
PubMed
29437550 Full text @ Zebrafish
Abstract
We have developed a simple method for the induction of cataracts in adult zebrafish using hydrogen peroxide. Our method uses a corneal puncture followed by intraocular injection of hydrogen peroxide into the anterior chamber. The procedure can be done rapidly, forming a highly opaque cataract almost immediately, and fish recover quickly. The optical clarity of the lens is gradually regained over time, showing the innate ability of zebrafish to regenerate healthy lens tissue. This technique provides a simple in vivo system with which to assess mutant strains or targeted disruptions to genes expressed in the lens.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes