PUBLICATION
Trek2a regulates gnrh3 expression under control of melatonin receptor Mt1 and α2-adrenoceptor.
- Authors
- Loganathan, K., Moriya, S., Parhar, I.S.
- ID
- ZDB-PUB-180206-1
- Date
- 2018
- Source
- Biochemical and Biophysical Research Communications 496(3): 927-933 (Journal)
- Registered Authors
- Keywords
- Adrenoceptor, GnRH, Melatonin, Reproduction, TREK
- MeSH Terms
-
- Animals
- Brain/metabolism*
- Cells, Cultured
- Gene Expression Regulation/physiology
- Gonadotropin-Releasing Hormone/metabolism*
- Male
- Melatonin/metabolism*
- Potassium Channels, Tandem Pore Domain/metabolism*
- Pyrrolidonecarboxylic Acid/analogs & derivatives*
- Pyrrolidonecarboxylic Acid/metabolism
- Receptor, Melatonin, MT1/metabolism*
- Receptors, Adrenergic, alpha-2/metabolism*
- Signal Transduction/physiology
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 29395088 Full text @ Biochem. Biophys. Res. Commun.
Citation
Loganathan, K., Moriya, S., Parhar, I.S. (2018) Trek2a regulates gnrh3 expression under control of melatonin receptor Mt1 and α2-adrenoceptor.. Biochemical and Biophysical Research Communications. 496(3):927-933.
Abstract
Gonadotrophin-releasing hormone (GnRH) expression is associated with the two-pore domain potassium ion (K+) channel-related K+ (TREK) channel trek2a expression and melatonin levels. We aimed to investigate correlation of trek2a expression with gnrh3 expression, and regulatory mechanisms of trek2a expression by the melatonin receptor Mt1 and α2-adrenoceptor which are regulated by melatonin. trek2a specific siRNA, Mt1 antagonist luzindole and α2-adrenoceptor antagonist prazosin were administered into the adult zebrafish brain and gene expressions were examined by real-time PCR. trek2a specific siRNA administration significantly reduced expression levels of trek2a, gnrh3 and mt1. Luzindole administration suppressed trek2a and gnrh3 expressions. Prazosin administration reduced trek2a and gnrh3 expressions. It is suggested that Trek2a regulates gnrh3 expression under the control of Mt1 and α2-adrenoceptor.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping