PUBLICATION
Activating the regenerative potential of Müller glia cells in a regeneration-deficient retina
- Authors
- Lust, K., Wittbrodt, J.
- ID
- ZDB-PUB-180130-11
- Date
- 2018
- Source
- eLIFE 7: (Journal)
- Registered Authors
- Wittbrodt, Jochen
- Keywords
- developmental biology, stem cells, zebrafish
- MeSH Terms
-
- Cell Proliferation
- Regeneration*
- Retina/injuries
- Retina/physiology*
- Animals
- Oryzias/physiology*
- Zebrafish/physiology*
- Cellular Reprogramming
- Ependymoglial Cells/physiology*
- PubMed
- 29376827 Full text @ Elife
Citation
Lust, K., Wittbrodt, J. (2018) Activating the regenerative potential of Müller glia cells in a regeneration-deficient retina. eLIFE. 7.
Abstract
Regeneration responses in animals are widespread across phyla. To identify molecular players that confer regenerative capacities to non-regenerative species is of key relevance for basic research and translational approaches. Here, we report a differential response in retinal regeneration between medaka (Oryzias latipes) and zebrafish (Danio rerio). In contrast to zebrafish, medaka Müller glia (olMG) cells behave like progenitors and exhibit a restricted capacity to regenerate the retina. After injury, olMG cells proliferate but fail to self-renew and ultimately only restore photoreceptors. In our injury paradigm, we observed that in contrast to zebrafish, proliferating olMG cells do not maintain sox2 expression. Sustained sox2 expression in olMG cells confers regenerative responses similar to those of zebrafish MG (drMG) cells. We show that a single, cell-autonomous factor reprograms olMG cells and establishes a regeneration-like mode. Our results position medaka as an attractive model to delineate key regeneration factors with translational potential.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping