|ZFIN ID: ZDB-PUB-180127-5|
The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio).
Usui, T., Noble, D.W.A., O'Dea, R.E., Fangmeier, M.L., Lagisz, M., Hesselson, D., Nakagawa, S.
|Source:||PeerJ 6: e4292 (Journal)|
|Registered Authors:||Hesselson, Daniel|
|Keywords:||Burst swimming, Circular, Endurance test, Sustained swimming, Technique|
|PubMed:||29372124 Full text @ Peer J.|
Usui, T., Noble, D.W.A., O'Dea, R.E., Fangmeier, M.L., Lagisz, M., Hesselson, D., Nakagawa, S. (2018) The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio).. PeerJ. 6:e4292.
ABSTRACTZebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.
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