PUBLICATION
The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio).
- Authors
- Usui, T., Noble, D.W.A., O'Dea, R.E., Fangmeier, M.L., Lagisz, M., Hesselson, D., Nakagawa, S.
- ID
- ZDB-PUB-180127-5
- Date
- 2018
- Source
- PeerJ 6: e4292 (Journal)
- Registered Authors
- Hesselson, Daniel
- Keywords
- Burst swimming, Circular, Endurance test, Sustained swimming, Technique
- MeSH Terms
- none
- PubMed
- 29372124 Full text @ Peer J.
Citation
Usui, T., Noble, D.W.A., O'Dea, R.E., Fangmeier, M.L., Lagisz, M., Hesselson, D., Nakagawa, S. (2018) The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio).. PeerJ. 6:e4292.
Abstract
Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping