ZFIN ID: ZDB-PUB-180127-5
The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio).
Usui, T., Noble, D.W.A., O'Dea, R.E., Fangmeier, M.L., Lagisz, M., Hesselson, D., Nakagawa, S.
Date: 2018
Source: PeerJ   6: e4292 (Journal)
Registered Authors: Hesselson, Daniel
Keywords: Burst swimming, Circular, Endurance test, Sustained swimming, Technique
MeSH Terms: none
PubMed: 29372124 Full text @ Peer J.
ABSTRACT
Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.
ADDITIONAL INFORMATION No data available