ZFIN ID: ZDB-PUB-180114-7
Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos
Xin, Y., Duan, C.
Date: 2018
Source: Methods in molecular biology (Clifton, N.J.)   1742: 205-211 (Chapter)
Registered Authors: Duan, Cunming
Keywords: CRISPR/Cas9, Gene knockdown, Microinjection, Morpholinos, Transgenesis, Zebrafish embryos, mRNA
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems
  • Embryonic Development
  • Female
  • Gene Expression Regulation, Developmental
  • Genetic Engineering/instrumentation*
  • Microinjections/methods*
  • Models, Animal
  • Morpholinos/administration & dosage
  • Oligonucleotides, Antisense/administration & dosage
  • RNA, Guide/administration & dosage
  • Ribonucleoproteins/administration & dosage
  • Thionucleotides
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zygote/growth & development
PubMed: 29330802 Full text @ Meth. Mol. Biol.
In this chapter, we describe a stepwise protocol of microinjection. Using this method, antisense morpholinos, CRISPR-Cas9 ribonucleoprotein complexes, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.