PUBLICATION
Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos
- Authors
- Xin, Y., Duan, C.
- ID
- ZDB-PUB-180114-7
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1742: 205-211 (Chapter)
- Registered Authors
- Duan, Cunming
- Keywords
- CRISPR/Cas9, Gene knockdown, Microinjection, Morpholinos, Transgenesis, Zebrafish embryos, mRNA
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems
- Embryonic Development
- Female
- Gene Expression Regulation, Developmental
- Genetic Engineering/instrumentation*
- Microinjections/methods*
- Models, Animal
- Morpholinos/administration & dosage
- Oligonucleotides, Antisense/administration & dosage
- RNA, Guide, Kinetoplastida/administration & dosage
- Ribonucleoproteins/administration & dosage
- Thionucleotides
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zygote/growth & development
- PubMed
- 29330802 Full text @ Meth. Mol. Biol.
Citation
Xin, Y., Duan, C. (2018) Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 1742:205-211.
Abstract
In this chapter, we describe a stepwise protocol of microinjection. Using this method, antisense morpholinos, CRISPR-Cas9 ribonucleoprotein complexes, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping