ZFIN ID: ZDB-PUB-180114-6
Hypoxic Treatment of Zebrafish Embryos and Larvae
Kamei, H., Duan, C.
Date: 2018
Source: Methods in molecular biology (Clifton, N.J.)   1742: 195-203 (Chapter)
Registered Authors: Duan, Cunming
Keywords: Developmental timing, Dissolved oxygen, Embryo, Hypoxic water, Zebrafish
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/drug effects
  • Hypoxia*
  • Larva/growth & development
  • Models, Animal*
  • Zebrafish/embryology
  • Zebrafish/growth & development*
PubMed: 29330801 Full text @ Meth. Mol. Biol.
Zebrafish has emerged as an informative animal model to study the biological impact and molecular mechanisms of hypoxia. Here we describe a simple method to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and can be applied to other fish and amphibian species.