PUBLICATION
High throughput in vitro and in vivo screening of inland waters of Southern California
- Authors
- Mehinto, A.C., VanDervort, D.R., Lao, W., He, G., Denison, M.S., Vliet, S.M., Volz, D.C., Mazor, R.D., Maruya, K.A.
- ID
- ZDB-PUB-180109-9
- Date
- 2017
- Source
- Environmental science. Processes & impacts 19: 1142-1149 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- California
- Embryo, Nonmammalian/drug effects
- Environmental Monitoring/methods*
- HEK293 Cells
- High-Throughput Screening Assays/methods*
- Humans
- Polycyclic Aromatic Hydrocarbons/analysis
- Polycyclic Aromatic Hydrocarbons/toxicity*
- Receptors, Aryl Hydrocarbon/metabolism
- Receptors, Estrogen/metabolism
- Receptors, Glucocorticoid/metabolism
- Rivers/chemistry*
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- PubMed
- 28612856 Full text @ Environ Sci Process Impacts
Citation
Mehinto, A.C., VanDervort, D.R., Lao, W., He, G., Denison, M.S., Vliet, S.M., Volz, D.C., Mazor, R.D., Maruya, K.A. (2017) High throughput in vitro and in vivo screening of inland waters of Southern California. Environmental science. Processes & impacts. 19:1142-1149.
Abstract
The impact of unmonitored contaminants, also known as contaminants of emerging concern (CECs), on freshwater streams remains largely uncharacterized. Water samples from 31 streams representing urban, agricultural and undeveloped (i.e., open space) land use in Southern California (USA) were analyzed for in vitro and in vivo bioactivity. The extent and magnitude of bioactivity screened using endocrine-responsive cell bioassays and a fish embryo screening assay were low. In contrast, a wider gradient of responses for the aryl hydrocarbon receptor (AhR) assay was observed, which was negatively correlated with a measure of benthic community structure. Both aromatic and non-aromatic CECs were tentatively identified in these samples, but polycyclic aromatic hydrocarbons (PAHs), known AhR agonists in urban environments, were not present at detectable levels. These results suggest that a combination of in vitro and in vivo show potential as screening techniques for biological condition in situ, but that more advanced, comprehensive analytical methods are needed to identify bioactive contaminants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping