PUBLICATION
Lithium promotes the production of reactive oxygen species via GSK-3β/TSC2/TOR signaling in the gill of zebrafish (Danio rerio)
- Authors
- Liu, D., Gao, L., Zhang, Z., Tao, S., Pang, Q., Li, A., Deng, H., Yu, H.
- ID
- ZDB-PUB-180102-7
- Date
- 2017
- Source
- Chemosphere 195: 854-863 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Glycogen synthase kinase-3β, Lithium, Reactive oxygen species, Target of rapamycin, Tuberous sclerosis complex 2
- MeSH Terms
-
- Animals
- Catalase/genetics
- Catalase/metabolism
- Gills/drug effects*
- Gills/metabolism
- Glutathione Peroxidase/genetics
- Glutathione Peroxidase/metabolism
- Glycogen Synthase Kinase 3 beta/genetics
- Glycogen Synthase Kinase 3 beta/metabolism
- Hydrogen Peroxide/metabolism
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism
- Lithium/pharmacology*
- Membrane Potential, Mitochondrial/drug effects
- Reactive Oxygen Species/metabolism*
- Signal Transduction/drug effects
- Superoxide Dismutase/genetics
- Superoxide Dismutase/metabolism
- TOR Serine-Threonine Kinases/genetics
- TOR Serine-Threonine Kinases/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 29291576 Full text @ Chemosphere
Citation
Liu, D., Gao, L., Zhang, Z., Tao, S., Pang, Q., Li, A., Deng, H., Yu, H. (2017) Lithium promotes the production of reactive oxygen species via GSK-3β/TSC2/TOR signaling in the gill of zebrafish (Danio rerio). Chemosphere. 195:854-863.
Abstract
In this study, the mechanism that lithium (Li) promotes the production of reactive oxygen species (ROS) via the glycogen synthase kinase-3β (GSK-3β)/tuberous sclerosis complex 2 (TSC2)/target of rapamycin (TOR) signaling was investigated in the gill of zebrafish (Danio rerio). After the zebrafish were treated by 25 and 50 mg/L Li+, the mRNA expression of GSK-3β and TSC2 was inhibited, but the expression of TOR was induced in the gill of zebrafish. The levels of hydrogen peroxide (H2O2), superoxide anion (O2·-), and hydroxy radical (·OH) as well as the activity of superoxide dismutase (SOD) were increased, while the activities of catalase (CAT), glutathione peroxidase (GSH-PX), and peroxidase (POD) were decreased by 25 and 50 mg/L Li+ treatments. In the ZF4 cells, the mRNA expression of GSK-3β and TSC2 was inhibited, but TOR expression was induced by 1, 5, and 10 mmol/L Li+ treatments. To further confirm that lithium promoted ROS production via GSK-3β inhibition, GSK-3β RNA was interfered. It was found that the interference of GSK-3β RNA induced the TSC2/TOR signaling. The levels of H2O2, O2·-, and ·OH were increased, but the activities of CAT, GSH-PX, and POD were decreased by GSK-3β RNA interference. In addition, lithium decreased the mitochondrial membrane potential (MMP) with Rhodamine-123 assay, but increased the levels of ROS by 2',7'-dichlorofluorescein diacetate (DCFH-DA) assay. The present results indicated that lithium promoted the ROS production through the GSK-3β/TSC2/TOR signaling in the gill of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping