PUBLICATION
Evaluation of toxicological endpoints in female zebrafish after bisphenol A exposure
- Authors
- Molina, A.M., Abril, N., Morales-Prieto, N., Monterde, J.G., Lora, A.J., Ayala, N., Moyano, R.
- ID
- ZDB-PUB-171221-4
- Date
- 2017
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 112: 19-25 (Journal)
- Registered Authors
- Keywords
- Aromatase, Atretic follicle, Bisphenol A, Vitellogenin, Zebrafish
- MeSH Terms
-
- Animals
- Aromatase/genetics
- Benzhydryl Compounds/toxicity*
- Biomarkers/metabolism
- Dose-Response Relationship, Drug
- Down-Regulation
- Endocrine Disruptors/toxicity*
- Environmental Monitoring/methods
- Estradiol/metabolism
- Female
- Follicular Atresia/drug effects*
- Ovary/drug effects*
- Ovary/enzymology
- Ovary/pathology
- Phenols/toxicity*
- RNA, Messenger/genetics
- Real-Time Polymerase Chain Reaction
- Up-Regulation
- Vitellogenins/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish*
- Zebrafish Proteins/genetics
- PubMed
- 29258955 Full text @ Food Chem. Toxicol.
- CTD
- 29258955
Citation
Molina, A.M., Abril, N., Morales-Prieto, N., Monterde, J.G., Lora, A.J., Ayala, N., Moyano, R. (2017) Evaluation of toxicological endpoints in female zebrafish after bisphenol A exposure. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 112:19-25.
Abstract
Given the importance of bisphenol A (BPA) as a xenoestrogen and its potential effects on human and animal health, we evaluated BPA exposure's short-term effects on follicular development, yolk protein vitellogenin (VTG) production and aromatase expression in female zebrafish. Histological modifications were observed along with increased presence of atretic follicles. Whole-body VTG concentration increased with the dose of BPA exposure. In contrast, expression of Cyp19a mRNA in the ovaries of BPA-exposed fish exhibited an apparent non-monotonic response curve, marked by downregulation at 1 μg/L BPA, upregulation at 10 μg/L BPA, and a return to downregulation at 100 μg/L BPA and higher doses. Ovaries only exhibited significant increases in follicular atresia and VTG concentration after exposure to 100 μg/L BPA and higher doses. Ovarian histopathology, aromatase Cyp19a transcript levels and whole-body VTG protein abundance may be good biomarkers for early detection of environmental BPA exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping