ZFIN ID: ZDB-PUB-171214-1
miR-34a Regulates Sperm Motility in Zebrafish
Guo, W., Xie, B., Xiong, S., Liang, X., Gui, J.F., Mei, J.
Date: 2017
Source: International Journal of Molecular Sciences   18(12): (Journal)
Registered Authors: Gui, Jian-Fang, Mei, Jie
Keywords: gsk3a, knockout, miR-34a, sperm motility
MeSH Terms:
  • 3' Untranslated Regions
  • Animals
  • Female
  • Fertilization
  • Gene Knockout Techniques
  • Glycogen Synthase Kinase 3/genetics*
  • Male
  • MicroRNAs/genetics*
  • Sexual Behavior, Animal
  • Sperm Motility
  • Spermatogenesis
  • Spermatozoa/physiology*
  • Zebrafish/genetics*
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
PubMed: 29232857 Full text @ Int. J. Mol. Sci.
Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system. miR-34a knockout zebrafish showed no obvious defects on testis morphology and sperm quantity. However, we found a significant increase in progressive sperm motility that is one of the pivotal factors influencing in vitro fertilization rates, in the knockout zebrafish. Moreover, breeding experiments showed that, when miR-34a-knockout male zebrafish mated with the wide-type females, they had a higher fertilization rate than did the wide-type males. Glycogen synthase kinase-3a (gsk3a), a potential sperm motility regulatory gene was predicted to be targeted by miR-34a, which was further supported by luciferase reporter assays, since a significant decrease of luciferase activity was detected upon ectopic overexpression of miR-34a. Our findings suggest that miR-34a downregulates gsk3a by targeting its 3' untranslated region, and miR-34a/gsk3a interaction modulates sperm motility in zebrafish. This study will help in understanding in the role of the miR-34 family during spermatogenesis and will set paths for further studies.