PUBLICATION

Transcriptional inhibition of TCDD-mediated induction of cytochrome P450 1A1 and alteration of protein expression in a zebrafish hepatic cell line following the administration of TCDD and Cd².

Authors

Chen, Y.Y., Chan, K.M.

ID

ZDB-PUB-171107-7

Date

2017

Source

Toxicology letters 282: 121-135 (Journal)

Registered Authors

Chan, King-Ming

Keywords

Aryl hydrocarbon receptor, Gene transcription, Proteomics

MeSH Terms

Animals
Cadmium Chloride/toxicity*
Cell Line
Cell Survival/drug effects
Cytochrome P-450 CYP1A1/biosynthesis*
Dose-Response Relationship, Drug
Environmental Pollutants/toxicity*
Enzyme Induction
Gene Expression/drug effects*
Liver/drug effects*
Liver/enzymology
Polychlorinated Dibenzodioxins/toxicity*
Zebrafish
Zebrafish Proteins/genetics

PubMed

29107029 Full text @ Toxicol. Lett.

CTD

29107029

Abstract

We studied the effects of Cd²⁺ on TCDD-mediated induction of the cytochrome P450 1A1 (cyp1a1) gene using a zebrafish liver cell line (ZFL). Our results showed that Cd²⁺ inhibited the TCDD-mediated induction of the cyp1a1 protein, enzyme activity, and mRNA expression level. Cd²⁺ also down-regulated levels of the aryl hydrocarbon receptor (ahr2) and the aryl hydrocarbon receptor nuclear translocator 2b (arnt2b) mRNAs. Compared with TCDD (3nM) treatment alone, co-treatment with Cd²⁺ (0-30μM) and TCDD (3nM) significantly inhibited the activity of the luciferase reporter gene constructs harboring the distal promoter region (P-2626/-2009) of CYP1A1 and the synthetic 3XRE gene promoter. This indicates that Cd²⁺ decreased the level of TCDD-induced cyp1a1 through transcriptional inhibition. Proteomic analysis was also used to evaluate the effect of Cd²⁺ on TCDD-altered protein expression in ZFL cells. The identified proteins are mainly enzymes of the glycolysis pathway and proteasomes, and have anti-oxidative and anti-stress effects.

Genes / Markers

Figures

Expression

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Chen et al., 2017 ZDB-PUB-171107-7 PMID:29107029

Transcriptional inhibition of TCDD-mediated induction of cytochrome P450 1A1 and alteration of protein expression in a zebrafish hepatic cell line following the administration of TCDD and Cd2.

Chen et al., 2017

ZDB-PUB-171107-7
PMID:29107029

Transcriptional inhibition of TCDD-mediated induction of cytochrome P450 1A1 and alteration of protein expression in a zebrafish hepatic cell line following the administration of TCDD and Cd².