PUBLICATION
Chemical and thermal sensitivity of medaka TRPA1 analyzed in heterologous expression system
- Authors
- Oda, M., Saito, K., Hatta, S., Kubo, Y., Saitoh, O.
- ID
- ZDB-PUB-171019-23
- Date
- 2017
- Source
- Biochemical and Biophysical Research Communications 494(1-2): 194-201 (Journal)
- Registered Authors
- Keywords
- Channel, Neuron, Thermosensing, Transient receptor potential
- MeSH Terms
-
- Animals
- Caffeine/toxicity
- Cold Temperature
- Female
- Fish Proteins/genetics
- Fish Proteins/physiology*
- Hot Temperature
- In Vitro Techniques
- Isothiocyanates/toxicity
- Monoterpenes/toxicity
- Oocytes/drug effects
- Oocytes/metabolism
- Oryzias/genetics
- Oryzias/physiology*
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Sensation/genetics
- Sensation/physiology
- TRPA1 Cation Channel/genetics
- TRPA1 Cation Channel/physiology
- Thermosensing/genetics
- Thermosensing/physiology
- Transient Receptor Potential Channels/drug effects
- Transient Receptor Potential Channels/genetics
- Transient Receptor Potential Channels/physiology*
- Xenopus
- Zebrafish/genetics
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- ortho-Aminobenzoates/toxicity
- PubMed
- 29037810 Full text @ Biochem. Biophys. Res. Commun.
Citation
Oda, M., Saito, K., Hatta, S., Kubo, Y., Saitoh, O. (2017) Chemical and thermal sensitivity of medaka TRPA1 analyzed in heterologous expression system. Biochemical and Biophysical Research Communications. 494(1-2):194-201.
Abstract
TRPA1 of insects and several tetrapod vertebrates except for those of rodents have been reported to be activated by noxious chemicals and also by high temperature with a relatively clear threshold. We previously analyzed the characteristics of two TRPA1 paralogs of zebrafish (zTRPA1a, b) and demonstrated that zTRPA1a is specialized for chemical sensing while zTRPA1b responds to thermal stimulations, that zTRPA1b responds to both cold and heat stimuli, and that heat stimulation gradually activates zTRPA1b without a clear threshold. In the medaka genome, a single TRPA1 (olTRPA1) gene is present. To examine if functional properties of olTRPA1 are similar to TRPA1 of land animals or either of zTRPA1a or zTRPA1b, we isolated a TRPA1 cDNA from medaka and performed functional analyses. OlTRPA1 showed a sensitivity to four noxious chemicals (allyl isothiocyanate, caffeine, carvacrol, methyl anthranilate). We observed that cold stimulation does not activate olTRPA1, but heat stimulation gradually activates olTRPA1 with an unclear threshold. Results suggested that a single TRPA1 functions as a chemical and thermal sensor in medaka, and that a gradual heat-activation without clear threshold might be a common feature for TRPA1 of fish living in water.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping