Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes
- Biga, P.R., Latimer, M.N., Froehlich, J.M., Gabillard, J.C., Seiliez, I.
- Biology Open 6(11): 1720-1725 (Journal)
- Registered Authors
- Biga, Peggy R., Gabillard, Jean-Charles, Seiliez, Iban
- Atrophy, Autophagy, Epigenetic, Histone modification, Myotube, Zebrafish
- MeSH Terms
- 29025701 Full text @ Biol. Open
Biga, P.R., Latimer, M.N., Froehlich, J.M., Gabillard, J.C., Seiliez, I. (2017) Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes. Biology Open. 6(11):1720-1725.
The zebrafish (Danio rerio) remains the teleost fish of choice for biological investigations due to the vast array of molecular tools and resources available. To better understand the epigenetic regulation of autophagy, we utilized a primary myotube culture system generated from isolated myogenic precursor cells (MPCs) from zebrafish grown under starvation conditions using a media devoid of serum and amino acids. Here, we report starvation-induced regulation of several autophagy-related genes (atg) expression and profile the distribution of H3K27me3, H3K9me3, and H3K4me3 marks along lc3b, atg4b and p62/sqstm1 loci. These data support epigenetic regulation of autophagy in response to starvation that suggests a level of regulation that can be sustained for chronic conditions via chromatin modification.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes