PUBLICATION
Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos
- Authors
- Parolini, M., Bini, L., Magni, S., Rizzo, A., Ghilardi, A., Landi, C., Armini, A., Del Giacco, L., Binelli, A.
- ID
- ZDB-PUB-171011-9
- Date
- 2018
- Source
- Environmental pollution (Barking, Essex : 1987) 232: 603-614 (Journal)
- Registered Authors
- Del Giacco, Luca, Ghilardi, Anna
- Keywords
- Benzoylecgonine, Cocaine, Ecgonine methyl ester, Illicit drugs, Proteomics, Zebrafish embryos
- MeSH Terms
-
- Animals
- Cocaine/analogs & derivatives
- Cocaine/metabolism
- Cocaine/toxicity*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/physiology
- Fresh Water
- Illicit Drugs/toxicity*
- Oxidation-Reduction
- Oxidative Stress/drug effects
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism*
- PubMed
- 28993024 Full text @ Environ. Pollut.
Citation
Parolini, M., Bini, L., Magni, S., Rizzo, A., Ghilardi, A., Landi, C., Armini, A., Del Giacco, L., Binelli, A. (2018) Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos. Environmental pollution (Barking, Essex : 1987). 232:603-614.
Abstract
Illicit drugs have been identified as emerging aquatic pollutants because of their widespread presence in freshwaters and potential toxicity towards aquatic organisms. Among illicit drug residues, cocaine (COC) and its main metabolites, namely benzoylecgonine (BE) and ecgonine methyl ester (EME), are commonly detected in freshwaters worldwide at concentration that can induce diverse adverse effects to non-target organisms. However, the information of toxicity and mechanisms of action (MoA) of these drugs, mainly of COC metabolites, to aquatic species is still fragmentary and inadequate. Thus, this study was aimed at investigating the toxicity of two concentrations (0.3 and 1.0 μg/L) of COC, BE and EME similar to those found in aquatic ecosystems on zebrafish (Danio rerio) embryos at 96 h post fertilization through a functional proteomics approach. Exposure to COC and both its metabolites significantly altered the protein profile of zebrafish embryos, modulating the expression of diverse proteins belonging to different functional classes, including cytoskeleton, eye constituents, lipid transport, lipid and energy metabolism, and stress response. Expression of vitellogenins and crystallins was modulated by COC and both its main metabolites, while only BE and EME altered proteins related to lipid and energy metabolism, as well as to oxidative stress response. Our data confirmed the potential toxicity of low concentrations of COC, BE and EME, and helped to shed light on their MoA on an aquatic vertebrate during early developmental period.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping