|ZFIN ID: ZDB-PUB-170912-8|
Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish
Suarez-Bregua, P., Chien, C.J., Megias, M., Du, S.D., Rotllant, J.
|Source:||Developmental dynamics : an official publication of the American Association of Anatomists 246(12): 992-1000 (Journal)|
|Registered Authors:||Du, Shao Jun (Jim), Rotllant, Josep|
|Keywords:||cardiac muscle, mef2, muscle-specificity, myod, skeletal muscle, transgenic zebrafish|
|PubMed:||28891223 Full text @ Dev. Dyn.|
Suarez-Bregua, P., Chien, C.J., Megias, M., Du, S.D., Rotllant, J. (2017) Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 246(12):992-1000.
Background Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown.
Results The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in-vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter zebrafish lines generated replicate the endogenous mustn1b expression pattern in early zebrafish embryos. Specific site direct-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions each one corresponding to a specific functional transcription factor binding site.
Conclusions Our data indicates that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2 kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factors (TFs). This article is protected by copyright. All rights reserved.