PUBLICATION
Protein Sam68 regulates the alternative splicing of survivin DEx3
- Authors
- Gaytan-Cervantes, J., Gonzalez-Torres, C., Maldonado, V., Zampedri, C., Ceballos-Cancino, G., Melendez-Zajgla, J.
- ID
- ZDB-PUB-170907-11
- Date
- 2017
- Source
- The Journal of biological chemistry 292: 13745-13757 (Journal)
- Registered Authors
- Keywords
- KHDRBS1, Sam68, alternative splicing, cancer, cancer biology, gene regulation, survivin, survivin DEx3
- MeSH Terms
-
- CRISPR-Cas Systems
- Protein Isoforms/antagonists & inhibitors
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA, Neoplasm/chemistry
- RNA, Neoplasm/metabolism*
- Tumor Burden
- RNA Interference
- Animals
- Mutagenesis, Site-Directed
- DNA-Binding Proteins/antagonists & inhibitors
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- RNA, Messenger/chemistry
- RNA, Messenger/metabolism*
- Exons
- Alternative Splicing*
- Gene Deletion
- Humans
- Transplantation, Heterologous
- Neoplasm Transplantation/pathology
- Recombinant Proteins/chemistry
- Recombinant Proteins/metabolism
- RNA-Binding Proteins/antagonists & inhibitors
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism*
- Clone Cells
- Embryo, Nonmammalian
- HeLa Cells
- Neoplasm Proteins/antagonists & inhibitors
- Neoplasm Proteins/genetics
- Neoplasm Proteins/metabolism*
- Inhibitor of Apoptosis Proteins/genetics
- Inhibitor of Apoptosis Proteins/metabolism*
- Response Elements*
- Point Mutation
- Adaptor Proteins, Signal Transducing/antagonists & inhibitors
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/metabolism*
- Zebrafish
- PubMed
- 28655776 Full text @ J. Biol. Chem.
Citation
Gaytan-Cervantes, J., Gonzalez-Torres, C., Maldonado, V., Zampedri, C., Ceballos-Cancino, G., Melendez-Zajgla, J. (2017) Protein Sam68 regulates the alternative splicing of survivin DEx3. The Journal of biological chemistry. 292:13745-13757.
Abstract
Messenger RNA alternative splicing (AS) regulates the expression of a variety of genes involved in both physiological and pathological processes. AS of the anti-apoptotic and proliferation-associated survivin (BIRC5) gene generates six isoforms, which regulate key aspects of cancer initiation and progression. One of the isoforms is survivin DEx3, in which the exclusion of exon 3 generates a unique carboxyl terminus with specific anti-apoptotic functions. This isoform is highly expressed in advanced stages of breast and cervical tumors. Therefore, understanding the mechanisms that regulate survivin DEx3 mRNA AS is clearly important. To this end, we designed a minigene (M), and in combination with a series of deletions and site-directed mutations, we determined that the first 22 bp of exon 3 contain cis-acting elements that enhance the exclusion of exon 3 to generate the survivin DEx3 mRNA isoform. Furthermore, using pulldown assays, we discovered that Sam68 is a possible trans-acting factor that binds to this region and regulates exon 3 splicing. This result was corroborated using a cell line in which the Sam68 binding site in the survivin gene was mutated with the CRISPR/Cas system. This work provides the first clues regarding the regulation of survivin DEx3 mRNA splicing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping