PUBLICATION
Ultraconserved Sequences Associated with HoxD Cluster Have Strong Repression Activity
- Authors
- Kushawah, G., Mishra, R.K.
- ID
- ZDB-PUB-170902-5
- Date
- 2017
- Source
- Genome biology and evolution 9: 2049-2054 (Journal)
- Registered Authors
- Mishra, Rakesh
- Keywords
- HoxD, enhancers, repressors, ultraconserved DNA sequences, vertebrates
- MeSH Terms
-
- Animals
- Base Sequence
- Cell Line
- Conserved Sequence
- Enhancer Elements, Genetic
- Genes, Reporter
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism
- Humans
- Mice
- Multigene Family*
- Repressor Proteins/genetics
- Repressor Proteins/metabolism
- Transfection
- Zebrafish Proteins/genetics
- PubMed
- 28859354 Full text @ Genome Biol. Evol.
Citation
Kushawah, G., Mishra, R.K. (2017) Ultraconserved Sequences Associated with HoxD Cluster Have Strong Repression Activity. Genome biology and evolution. 9:2049-2054.
Abstract
Increase in the complexity of organisms during evolution strongly correlates with the increase in the noncoding DNA content of their genomes. Although a gradual increase in the proportion of repetitive DNA elements along with increasing complexity is known, most of the noncoding components of the genome remain uncharacterized. A nonrepetitive but highly conserved noncoding component of the genome in vertebrates, called ultraconserved DNA sequences, constitutes up to 5% of the human genome. The function of most of the ultraconserved DNA elements is not well known. One such ultraconserved stretch of DNA has been identified upstream of the HoxD cluster in vertebrates. We analyzed the function of these elements in different cell lines and zebrafish. Our results suggest that these ultraconserved sequences work as repressor elements. This is the first report which reveals the repressor function of ultraconserved sequences and implicates their role in the regulation of developmental genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping