PUBLICATION

Atrazine exposure decreases the activity of DNMTs, global DNA methylation levels, and dnmt expression

Authors
Wirbisky-Hershberger, S.E., Sanchez, O.F., Horzmann, K.A., Thanki, D., Yuan, C., Freeman, J.L.
ID
ZDB-PUB-170902-4
Date
2017
Source
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association   109(Pt 1): 727-734 (Journal)
Registered Authors
Freeman, Jennifer
Keywords
Atrazine, Epigenetics, Methylation, Zebrafish, dnmt
MeSH Terms
  • Animals
  • Atrazine/toxicity*
  • DNA Methylation/drug effects*
  • DNA-Cytosine Methylases/genetics*
  • DNA-Cytosine Methylases/metabolism
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Female
  • Gene Expression Regulation, Developmental
  • Herbicides/toxicity*
  • Male
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
28859886 Full text @ Food Chem. Toxicol.
Abstract
Atrazine, a herbicide used on agricultural crops is widely applied in the Midwestern United States as well as other areas of the globe. Atrazine frequently contaminates potable water supplies and is a suspected endocrine disrupting chemical. Previous studies have reported morphological, hormonal, and molecular alterations due to developmental and adulthood atrazine exposure; however, studies examining epigenetic alterations are limited. In this study, the effects of atrazine exposure on DNA methyltransferase (DNMT) activity and kinetics were evaluated. Global DNA methylation levels and dnmt expression in zebrafish larvae exposed to 0, 3, or 30 parts per billion (ppb) atrazine throughout embryogenesis was then assessed. Results indicate that atrazine significantly decreased the activity of maintenance DNMTs and that the inhibition mechanism can be described using non-competitive Michaelis-Menten kinetics. Furthermore, results show that an embryonic atrazine exposure decreases global methylation levels and the expression of dnmt4 and dnmt5. These findings indicate that atrazine exposure can decrease the expression and activity of DNMTs, leading to decreased DNA methylation levels.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes