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ZFIN ID: ZDB-PUB-170829-4
Differential regulation of the duplicated fabp7, fabp10 and fabp11 genes of zebrafish by peroxisome proliferator activated receptors
Laprairie, R.B., Denovan-Wright, E.M., Wright, J.M.
Date: 2017
Source: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   213: 81-90 (Journal)
Registered Authors: Wright, Jonathan M.
Keywords: none
MeSH Terms:
  • Animals
  • Fatty Acid-Binding Protein 7/biosynthesis*
  • Fatty Acid-Binding Protein 7/genetics
  • Fatty Acid-Binding Proteins/biosynthesis*
  • Fatty Acid-Binding Proteins/genetics
  • Gene Duplication*
  • Gene Expression Regulation/physiology*
  • HEK293 Cells
  • Humans
  • NF-kappa B p50 Subunit/genetics
  • NF-kappa B p50 Subunit/metabolism
  • Peroxisome Proliferator-Activated Receptors/genetics
  • Peroxisome Proliferator-Activated Receptors/metabolism*
  • Promoter Regions, Genetic/physiology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
PubMed: 28844908 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
In the duplication-degeneration-complementation model, duplicated gene-pairs undergo nonfunctionalization (loss from the genome), subfunctionalization (the functions of the ancestral gene are sub-divided between duplicate genes), or neofunctionalization (one of the duplicate genes acquires a new function). These processes occur by loss or gain of regulatory elements in gene promoters. Fatty acid-binding proteins (Fabp) belong to a multigene family composed of orthologous proteins that are highly conserved in sequence and function, but differ in their gene regulation. We previously reported that the zebrafish fabp1a, fabp1b.1, and fabp1b.2 promoters underwent subfunctionalization of PPAR responsiveness. Here, we describe the regulation at the duplicated zebrafish fabp7a/fabp7b, fabp10a/fabp10b and fabp11a/fabp11b gene promoters. Differential control at the duplicated fabp promoters was assessed by DNA sequence analysis, responsiveness to PPAR-isoform specific agonists and NF-κB p50 antagonists in zebrafish liver and intestine explant tissue, and in HEK293A cells transfected with fabp promoter-reporter constructs. Each zebrafish fabp gene displayed unique transcriptional regulation compared to its paralogous duplicate. This work provides a framework to account for the evolutionary trajectories that led to the high retention (57%) of duplicated fabp genes in the zebrafish genome compared to only ~3% of all duplicated genes in the zebrafish genome.