ZFIN ID: ZDB-PUB-170818-3
The loss and recovery of vertebrate vision examined in microplates
Thorn, R.J., Clift, D.E., Ojo, O., Colwill, R.M., Creton, R.
Date: 2017
Source: PLoS One   12: e0183414 (Journal)
Registered Authors: Creton, Robbert
Keywords: Larvae, Zebrafish, Morpholino, Swimming, Vision, Photoreceptors, Embryos, Vertebrates
MeSH Terms:
  • Animals
  • Automation
  • Cyclic Nucleotide Phosphodiesterases, Type 6/genetics
  • Gene Knockdown Techniques
  • Ultraviolet Rays
  • Vertebrates/physiology*
  • Vision Disorders/enzymology
  • Vision Disorders/physiopathology
  • Vision, Ocular*
  • Zebrafish/physiology*
PubMed: 28817700 Full text @ PLoS One
Regenerative medicine offers potentially ground-breaking treatments of blindness and low vision. However, as new methodologies are developed, a critical question will need to be addressed: how do we monitor in vivo for functional success? In the present study, we developed novel behavioral assays to examine vision in a vertebrate model system. In the assays, zebrafish larvae are imaged in multiwell or multilane plates while various red, green, blue, yellow or cyan objects are presented to the larvae on a computer screen. The assays were used to examine a loss of vision at 4 or 5 days post-fertilization and a gradual recovery of vision in subsequent days. The developed assays are the first to measure the loss and recovery of vertebrate vision in microplates and provide an efficient platform to evaluate novel treatments of visual impairment.