PUBLICATION
Hydrogen peroxide regulates angiogenesis-related factors in tumor cells
- Authors
- Jerónimo, A., Rodrigues, G., Vilas-Boas, F., Martins, G.G., Bagulho, A., Real, C.
- ID
- ZDB-PUB-170818-2
- Date
- 2017
- Source
- Biochemistry and cell biology = Biochimie et biologie cellulaire 95(6): 679-685 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Angiogenesis Inducing Agents/metabolism*
- Animals
- Catalase/genetics
- Catalase/metabolism*
- Cell Line, Tumor
- Humans
- Hydrogen Peroxide/pharmacology*
- Mice
- Neoplasms/metabolism*
- Neoplasms/pathology
- Neovascularization, Pathologic/metabolism*
- Neovascularization, Pathologic/pathology
- Zebrafish/embryology
- PubMed
- 28817784 Full text @ Biochem. Cell Biol.
Citation
Jerónimo, A., Rodrigues, G., Vilas-Boas, F., Martins, G.G., Bagulho, A., Real, C. (2017) Hydrogen peroxide regulates angiogenesis-related factors in tumor cells. Biochemistry and cell biology = Biochimie et biologie cellulaire. 95(6):679-685.
Abstract
Tumor angiogenesis is required for tumor development and growth, and is regulated by several factors including ROS. H2O2 is a ROS with an important role in cell signaling, but how H2O2 regulates tumor angiogenesis is still poorly understood. We have xenografted tumor cells with altered levels of H2O2 by catalase overexpression into zebrafish embryos to study redox-induced tumor neovascularization. We found that vascular recruitment and invasion were impaired if catalase was overexpressed. In addition, the overexpression of catalase altered the transcriptional levels of several angiogenesis-related factors in tumor cells, including TIMP-3 and THBS1. These two anti-angiogenic factors were found to be H2O2-regulated by two different mechanisms: TIMP-3 expression in a cell-autonomous manner; and, THBS1 expression that was non-cell-autonomous. Our work shows that intracellular H2O2 regulates the expression of angiogenic factors and the formation of a vessel network. Understanding the molecular mechanisms that govern this multifunctional effect of H2O2 on tumor angiogenesis could be important for the development of more efficient anti-angiogenic therapies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping