ZFIN ID: ZDB-PUB-170726-20
Conformational Changes of CFTR upon Phosphorylation and ATP Binding
Zhang, Z., Liu, F., Chen, J.
Date: 2017
Source: Cell 170(3): 483-491.e8 (Journal)
Registered Authors:
Keywords: ABC transporter, ATP-bound, CFTR, anion channel, cryo-EM, phosphorylated form
MeSH Terms:
  • Adenosine Triphosphate/metabolism
  • Amino Acid Sequence
  • Cryoelectron Microscopy
  • Cystic Fibrosis Transmembrane Conductance Regulator/chemistry*
  • Cystic Fibrosis Transmembrane Conductance Regulator/metabolism
  • Humans
  • Models, Molecular
  • Protein Domains
  • Sequence Alignment
  • Zebrafish Proteins/chemistry*
  • Zebrafish Proteins/metabolism
PubMed: 28735752 Full text @ Cell
The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel evolved from an ATP-binding cassette transporter. CFTR channel gating is strictly coupled to phosphorylation and ATP hydrolysis. Previously, we reported essentially identical structures of zebrafish and human CFTR in the dephosphorylated, ATP-free form. Here, we present the structure of zebrafish CFTR in the phosphorylated, ATP-bound conformation, determined by cryoelectron microscopy to 3.4 Å resolution. Comparison of the two conformations shows major structural rearrangements leading to channel opening. The phosphorylated regulatory domain is disengaged from its inhibitory position; the nucleotide-binding domains (NBDs) form a "head-to-tail" dimer upon binding ATP; and the cytoplasmic pathway, found closed off in other ATP-binding cassette transporters, is cracked open, consistent with CFTR's unique channel function. Unexpectedly, the extracellular mouth of the ion pore remains closed, indicating that local movements of the transmembrane helices can control ion access to the pore even in the NBD-dimerized conformation.