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ZFIN ID: ZDB-PUB-170726-11
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
Zhang, Y., Qin, W., Lu, X., Xu, J., Huang, H., Bai, H., Li, S., Lin, S.
Date: 2017
Source: Nature communications   8: 118 (Journal)
Registered Authors: Huang, Haigen, Lin, Shuo
Keywords: CRISPR-Cas9 genome editing, Genetic engineering, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CRISPR-Cas Systems/genetics*
  • Fetal Proteins/genetics
  • Gene Editing/methods*
  • Genetic Engineering/methods
  • Growth Differentiation Factor 6/genetics
  • Mutagenesis, Site-Directed/methods*
  • Point Mutation
  • Reproducibility of Results
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • T-Box Domain Proteins/genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 28740134 Full text @ Nat. Commun.
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ABSTRACT
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity.
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